RT Journal Article
T1 Interlaboratory validation of a multiplex qPCR method for the detection of Listeria monocytogenes in a ready-to-eat seafood product
A1 Azinheiro, Sarah
A1 Rodríguez López, Pedro
A1 Lozano León, Antonio
A1 Guedes, Hugo
A1 Regal López, Patricia
A1 Franco Abuín, Carlos Manuel
A1 Cepeda Sáez, Alberto
A1 Teixeira, Pilar
A1 Rodrigues De Melo, Luís Daniel
A1 Silva, Daniela
A1 Fernández, Ana
A1 Faria, Marcia
A1 Roumani, Foteini
A1 Herrera, Juan
A1 Prado Rodríguez, Marta
A1 López Cabo, Marta
A1 Garrido Maestu, Alejandro
K1 Interlaboratory validation
K1 Listeria monocytogenes
K1 qPCR
K1 Ready-to-eat
K1 Fish products
K1 Alternative methods
AB Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10 CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as “almost complete agreement”.
PB Elsevier
SN 0956-7135
YR 2023
FD 2023-04-04
LK https://hdl.handle.net/10347/43472
UL https://hdl.handle.net/10347/43472
LA eng
NO Food Control 150 (2023) 109769
NO INTERREG EAPA_758/2018
DS Minerva
RD 3 may 2026