RT Journal Article
T1 A comprehensive coding and microRNA transcriptome of vertebral bone in postlarvae and juveniles of Senegalese sole (Solea senegalensis)
A1 Bouza Fernández, María Carmen
A1 Losada García, Ana Paula
A1 Fernández López, José Carlos
A1 Álvarez Dios, José Antonio
A1 De Azevedo, Ana Manuela
A1 Barreiro Lois, Andrés
A1 Costas, Damián
A1 Quiroga Berdeal, María Isabel
A1 Martínez Portela, Paulino
A1 Vázquez Rodríguez, Sonia
K1 Solea senegalensis
K1 Vertebral bone
K1 Spinal anomalies
K1 Transcriptomics
K1 mRNA
K1 miRNA
AB Understanding vertebral bone development is essential to prevent skeletal malformations in farmed fish related to genetic and environmental factors. This is an important issue in Solea senegalensis, with special impact of spinal anomalies in postlarval and juvenile stages. Vertebral bone transcriptomics in farmed fish mainly comes from coding genes, and barely on miRNA expression. Here, we used RNA-seq of spinal samples to obtain the first comprehensive coding and miRNA transcriptomic repertoire for postlarval and juvenile vertebral bone, covering different vertebral phenotypes and egg-incubation temperatures related to skeleton health in S. senegalensis. Coding genes, miRNA and pathways regulating bone development and growth were identified. Differential transcriptomic profiles and suggestive mRNA-miRNA interactions were found between postlarvae and juveniles. Bone-related genes and functions were associated with the extracellular matrix, development and regulatory processes, calcium binding, retinol and lipid metabolism or response to stimulus, including those revealed by the miRNA targets related to signaling, cellular and metabolic processes, growth, cell proliferation and biological adhesion. Pathway enrichment associated with fish skeleton were identified when comparing postlarvae and juveniles: growth and bone development functions in postlarvae, while actin cytoskeleton, focal adhesion and proteasome related to bone remodeling in juveniles. The transcriptome data disclosed candidate coding and miRNA gene markers related to bone cell processes, references for functional studies of the anosteocytic bone of S. senegalensis. This study establishes a broad transcriptomic foundation to study healthy and anomalous spines under early thermal conditions across life-stages in S. senegalensis, and for comparative analysis of skeleton homeostasis and pathology in fish and vertebrates
PB Elsevier
SN 0888-7543
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33866
UL http://hdl.handle.net/10347/33866
LA eng
NO Genomics, Volume 116, Issue 2, 2024, 110802
NO This study was supported by the Spanish Ministry of Science, Innovation and Universities, and European Regional Development Fund (RTI2018-097110-B-C21). AMdA has awarded a postdoctoral contract from Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, Spain. We thank Stolt Sea Farm S.A. for providing the egg samples, and the personal involved in fish culture and sampling at the Station of Marine Science of Toralla (ECIMAT, University of Vigo). We acknowledge the bioinformatic support of Andrés Hortas, Óscar Aramburu and Galicia Supercomputing Center (CESGA). Thanks to Lucía Ínsua and Sonia Gómez for technical and administrative support
DS Minerva
RD 28 abr 2026