RT Journal Article
T1 Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison
A1 Lamas Freire, Alexandre
A1 Regal López, Patricia
A1 Vázquez Belda, Beatriz Isabel
A1 Miranda López, José Manuel
A1 Cepeda Sáez, Alberto
A1 Franco Abuín, Carlos Manuel
K1 rbST
K1 Nanoliter PCR
K1 Gene expression
K1 Screening
K1 Blood
K1 Hair
K1 Somatic cells
AB The use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden in the European Union. Due to the very low circulating concentration of rbST in treated animals, its direct detection is still a challenge. Therefore, the use of indirect methods to detect the ab(use) of rbST in dairy cattle appears as a good alternative. In the past few years, gene expression demonstrated its utility in screening the use of illicit substances in both humans and animals. In this study, a comparison of three types of matrices (milk somatic cells, blood, and hair follicles) was carried out to evaluate their potential use for routine control of rbST using 15 gene-expression profiles. A total of six rbST-treated cows and three control cows were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group. Samples of the three matrices were collected before rbST administration, and at three and nine days after treatment. The quality of RNA extracted was higher in the blood and hair-follicle samples than in the milk somatic cells. In the three matrices, there were significant differences in the expression of some genes, with milk somatic cells and blood presenting the the best matrices. On this note, the cyclin D1 (CCND1), interleukin 1 beta (IL-1b), tumor necrosis factor (TNF), and insulin-like growth factor 1 receptor (IGF-1R) genes showed potential as biomarkers of rbST treatment. Therefore, blood, somatic cells, and follicle hair should be considered as promising sources of RNA, and can be used in gene-expression assays to routinely control the illicit use of rbST
PB MDPI
SN 1420-3049
YR 2018
FD 2018-07-13
LK http://hdl.handle.net/10347/17018
UL http://hdl.handle.net/10347/17018
LA eng
NO Lamas, A.; Regal, P.; Vazquez, B.; Miranda, J.M.; Cepeda, A.; Franco, C.M. Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison. Molecules 2018, 23, 1708.
NO Spanish Innovation Program “Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad” (project AGL2014-58881-R)
DS Minerva
RD 26 abr 2026