RT Journal Article
T1 Computer-Assisted Recovery of Threatened Plants: Keys for Breaking Seed Dormancy of Eryngium viviparum
A1 Ayuso, Manuel
A1 Ramil Rego, Pablo
A1 Landín Pérez, Mariana
A1 Gallego, Pedro P.
A1 Barreal, M. Esther
K1 Apiaceae
K1 Artificial intelligence
K1 Conservation
K1 Germination
K1 Dormancy
K1 Endemic plant
K1 Embryo seed ratio
K1 Underdeveloped embryo
AB Many endangered plants such as Eryngium viviparum (Apiaceae) present a poor germination rate. This fact could be due to intrinsic and extrinsic seed variability influencing germination and dormancy of seeds. The objective of this study is to better understand the physiological mechanism of seed latency and, through artificial intelligence models, to determine the factors that stimulate germination rates of E. viviparum seeds. This description could be essential to prevent the disappearance of endangered plants. Germination in vitro was carried out under different dormancy breaking and incubation procedures. Percentages of germination, viability and E:S ratio were calculated and seeds were dissected at the end of each assay to describe embryo development. The database obtained was modeled using neurofuzzy logic technology. We have found that the most of Eryngium seeds (62.6%) were non-viable seeds (fully empty or without embryos). Excluding those, we have established the germination conditions to break seed dormancy that allow obtaining a real germination rate of 100%. Advantageously, the best conditions pointed out by neurofuzzy logic model for embryo growth were the combination of 1 mg L−1 GA3 (Gibberellic Acid) and high incubation temperature and for germination the combination of long incubation and short warm stratification periods. Our results suggest that E. viviparum seeds present morphophysiological dormancy, which reduce the rate of germination. The knowledge provided by the neurofuzzy logic model makes possible not just break the physiological component of dormancy, but stimulate the embryo development increasing the rate of germination. Undoubtedly, the strategy developed in this work can be useful to recover other endangered plants by improving their germination rate and uniformity favoring their ex vitro conservation
PB Frontiers Media
YR 2017
FD 2017
LK http://hdl.handle.net/10347/21564
UL http://hdl.handle.net/10347/21564
LA eng
NO Ayuso M, Ramil-Rego P, Landin M, Gallego PP and Barreal ME (2017) Computer-Assisted Recovery of Threatened Plants: Keys for Breaking Seed Dormancy of Eryngium viviparum. Front. Plant Sci. 8:2092. doi: 10.3389/fpls.2017.02092
NO This research was supported/partially supported by TREMEDAL—Inland wetlands of Northern Iberian Peninsula: management and restoration of mires and wet environments European Union (LIFE11 NAT/ES/000707, 2012–2015) and “Red de Uso Sostenible de Recursos y Residuos” funded by XUNTA DE GALICIA (R2014/019)
DS Minerva
RD 28 abr 2026