RT Journal Article
T1 Applications of genotyping by sequencing in aquaculture breeding and genetics
A1 Robledo Sánchez, Diego
A1 Palaiokostas, Christos
A1 Bargelloni, Luca
A1 Martínez Portela, Paulino
A1 Houston, Ross D.
K1 Aquaculture
K1 Genotyping
K1 Next‐generation sequencing
K1 Restriction‐site associated DNA
K1 Selective breeding
K1 Single nucleotide polymorphism
AB Selective breeding is increasingly recognized as a key component of sustainable production of aquaculture species. The uptake of genomic technology in aquaculture breeding has traditionally lagged behind terrestrial farmed animals. However, the rapid development and application of sequencing technologies has allowed aquaculture to narrow the gap, leading to substantial genomic resources for all major aquaculture species. While high‐density single‐nucleotide polymorphism (SNP) arrays for some species have been developed recently, direct genotyping by sequencing (GBS) techniques have underpinned many of the advances in aquaculture genetics and breeding to date. In particular, restriction‐site associated DNA sequencing (RAD‐Seq) and subsequent variations have been extensively applied to generate population‐level SNP genotype data. These GBS techniques are not dependent on prior genomic information such as a reference genome assembly for the species of interest. As such, they have been widely utilized by researchers and companies focussing on nonmodel aquaculture species with relatively small research communities. Applications of RAD‐Seq techniques have included generation of genetic linkage maps, performing genome‐wide association studies, improvements of reference genome assemblies and, more recently, genomic selection for traits of interest to aquaculture like growth, sex determination or disease resistance. In this review, we briefly discuss the history of GBS, the nuances of the various GBS techniques, bioinformatics approaches and application of these techniques to various aquaculture species
PB Wiley
SN 1753-5123
YR 2018
FD 2018
LK http://hdl.handle.net/10347/24293
UL http://hdl.handle.net/10347/24293
LA eng
NO Robledo, D., Palaiokostas, C., Bargelloni, L., Martínez, P. and Houston, R. (2018), Applications of genotyping by sequencing in aquaculture breeding and genetics. Rev Aquacult, 10: 670-682. https://doi.org/10.1111/raq.12193
NO The authors are supported by funding from the European Union's Seventh Framework Programme (FP7 2007‐2013) under grant agreement no. 613611 (FISHBOOST) and no.: 311920 (AquaTrace), Biotechnology and Biological Science Research Council (BBSRC) grants (BB/N024044/1, BB/M028321/1), and by BBSRC Institute Strategic Funding Grants to The Roslin Institute (BB/J004235/1, BB/J004324/1)
DS Minerva
RD 29 abr 2026