RT Journal Article
T1 Surface Plasmon Resonance Biosensor Method for Palytoxin Detection Based on Na+,K+-ATPase Affinity
A1 Alfonso Rancaño, María Amparo
A1 Pazos Guldrís, María José
A1 Fernández Araujo, Andrea
A1 Tobío Ageitos, Araceli
A1 Alfonso, Carmen
A1 Botana López, Luis Miguel
A1 Rodríguez Vieytes, Mercedes
K1 Palytoxin
K1 Na+,K+-ATPase
K1 Surface plasmon resonance biosensor
K1 Ostreopsis siamensis
AB Palytoxin (PLTX), produced by dinoflagellates from the genus Ostreopsis was first discovered, isolated, and purified from zoanthids belonging to the genus Palythoa. The detection of this toxin in contaminated shellfish is essential for human health preservation. A broad range of studies indicate that mammalian Na+,K+-ATPase is a high affinity cellular receptor for PLTX. The toxin converts the pump into an open channel that stimulates sodium influx and potassium efflux. In this work we develop a detection method for PLTX based on its binding to the Na+,K+-ATPase. The method was developed by using the phenomenon of surface plasmon resonance (SPR) to monitor biomolecular reactions. This technique does not require any labeling of components. The interaction of PLTX over immobilized Na+,K+-ATPase is quantified by injecting different concentrations of toxin in the biosensor and checking the binding rate constant (kobs). From the representation of kobs versus PLTX concentration, the kinetic equilibrium dissociation constant (KD) for the PLTX-Na+,K+-ATPase association can be calculated. The value of this constant is KD = 6.38 × 10−7 ± 6.67 × 10−8 M PLTX. In this way the PLTX-Na+,K+-ATPase association was used as a suitable method for determination of the toxin concentration in a sample. This method represents a new and useful approach to easily detect the presence of PLTX-like compounds in marine products using the mechanism of action of these toxins and in this way reduce the use of other more expensive and animal based methods.
PB MDPI
YR 2013
FD 2013
LK http://hdl.handle.net/10347/23012
UL http://hdl.handle.net/10347/23012
LA eng
NO Alfonso, A., Pazos, M.-J., Fernández-Araujo, A., Tobio, A., Alfonso, C., Vieytes, M., & Botana, L. (2013). Surface Plasmon Resonance Biosensor Method for Palytoxin Detection Based on Na+,K+-ATPase Affinity. Toxins, 6(1), 96–107. MDPI AG. Retrieved from http://dx.doi.org/10.3390/toxins6010096
NO The research leading to these results has received funding from the following FEDER cofunded-grants: From Ministerio de Ciencia y Tecnología, Spain: AGL2009-13581-CO2-01, AGL2012-40485-CO2-01. From Xunta de Galicia, Spain: 10PXIB261254 PR.From the European Union’s Seventh Framework Programme managed by REA—Research Executive Agency http://ec.europa.eu/research/rea (FP7/2007–2013) under grant agreement Nos. 211326-CP (CONffIDENCE), 265896 BAMMBO, 265409 µAQUA, and 262649 BEADS, 315285 Ciguatools and 312184 PharmaSea. From the Atlantic Area Programme (Interreg IVB Trans-national): 2008-1/003 (Atlantox) and 2009-1/117 (Pharmatlantic)
DS Minerva
RD 22 abr 2026