RT Journal Article
T1 Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
A1 González Amado, Marlen
A1 Tavares, Ana Paula Mora
A1 Freire, Mara G.
A1 Soto Campos, Ana María
A1 Rodríguez Figueiras, Óscar
K1 Aqueous two-phase systems
K1 Cheese whey
K1 Bovine serum albumin
K1 β-lactoglobulin
K1 α-lactalbumin
K1 Separation
AB Cheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes.
PB Elsevier
YR 2020
FD 2020-09
LK https://hdl.handle.net/10347/44223
UL https://hdl.handle.net/10347/44223
LA eng
NO González-Amado, M., Tavares, A. P. M., Freire, M. G., Soto, A., & Rodríguez, O. (2021). Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems. Separation and Purification Technology, 255, 117686. https://doi.org/10.1016/j.seppur.2020.117686
NO This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES, and project Multibiorefinery (POCI-01-0145-FEDER-016403), financed by national funds through the FCT/MEC, and, when appropriate, co-financed by FEDER under the PT2020 Partnership Agreement. Ana P. M. Tavares acknowledges FCT for the research contract under the Investigator Programme and Exploratory Project (IF/01634/2015). Oscar Rodríguez acknowledges financial support from the Regional Government (Xunta de Galicia, Spain) through project ED431F 2017/05.
DS Minerva
RD 9 jun 2026