RT Journal Article
T1 Liquid chromatography tandem mass spectrometry determination of selected synthetic cathinones and two piperazines in oral fluid. Cross reactivity study with the Dräger DrugTest 5000
A1 Hadriana Fernández-Vega, 
A1 Stefan Steinmeyer, 
A1 Castro Ríos, Ana de
A1 Lendoiro Belío, Elena
A1 López-Rivadulla Lamas, Manuel
A1 Cruz Landeira, Angelines
K1 Synthethic cathinone
K1 Synthethic piperazine
K1 LC-MS/MS
K1 Inmunoassay cross reactivity
K1 Oral fluid
AB Since the past few years, several synthetic cathinones and piperazines have been introduced into the drug market to substitute illegal stimulant drugs such as amphetamine and derivatives or cocaine due to their unregulated situation. These emerging drugs are not usually included in routine toxicological analysis. We developed and validated a LC–MS/MS method for the determination of methedrone, methylone, mephedrone, 3,4-methylenedioxypyrovalerone (MDPV), fluoromethcathinone, fluoromethamphetamine, 1-(3-chlorophenyl)piperazine (mCPP) and 3-trifluoromethylphenylpiperazine (TFMPP) in oral fluid. Sample extraction was performed using Strata X cartridges. Chromatographic separation was achieved in 10 min using an Atlantis® T3 column (100 mm × 2.1 mm, 3 μm), and formic acid 0.1% and acetonitrile as mobile phase. The method was satisfactorily validated, including selectivity, linearity (0.2–0.5 to 200 ng/mL), limits of detection (0.025–0.1 ng/mL) and quantification (0.2–0.5 ng/mL), imprecision and accuracy in neat oral fluid (%CV = 0.0–12.7% and 84.8–103.6% of target concentration, respectively) and in oral fluid mixed with Quantisal™ buffer (%CV = 7.2–10.3% and 80.2–106.5% of target concentration, respectively), matrix effect in neat oral fluid (−11.6 to 399.7%) and in oral fluid with Quantisal™ buffer (−69.9 to 131.2%), extraction recovery (87.9–134.3%) and recovery from the Quantisal™ (79.6–107.7%), dilution integrity (75–99% of target concentration) and stability at different conditions (−14.8 to 30.8% loss). In addition, cross reactivity produced by the studied synthetic cathinones in oral fluid using the Dräger DrugTest 5000 was assessed. All the analytes produced a methamphetamine positive result at high concentrations (100 or 10 μg/mL), and fluoromethamphetamine also at low concentration (0.075 μg/mL).
PB Elsevier
SN 0021-9673
YR 2014
FD 2014-12-29
LK https://hdl.handle.net/10347/38886
UL https://hdl.handle.net/10347/38886
LA eng
NO de Castro, A., Lendoiro, E., Fernández-Vega, H., Steinmeyer, S., López-Rivadulla, M., & Cruz, A. (2014). Liquid chromatography tandem mass spectrometry determination of selected synthetic cathinones and two piperazines in oral fluid. Cross reactivity study with an on-site immunoassay device. Journal of Chromatography A, 1374, 93-101. https://doi.org/10.1016/J.CHROMA.2014.11.024
NO Dirección General de Tráfico (Ministerio del Interior, España)
NO Dräger Safety AG & Co. KgaA (Lübeck, Germany)
NO Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia
DS Minerva
RD 29 abr 2026