RT Journal Article
T1 Volumetric Absorptive Microsampling (VAMS) for assayingimmunosuppressants from venous whole blood by LC–MS/MS using anovel atmospheric pressure ionization probe (UniSprayTM)
A1 Paniagua-González, Lucía
A1 Díaz-Louzao, Carla
A1 Lendoiro Belío, Elena
A1 Otero-Antón, Esteban
A1 Cadarso Suárez, Carmen María
A1 López-Rivadulla Lamas, Manuel
A1 Cruz Landeira, Angelines
A1 Castro Ríos, Ana de
K1 Immunosuppressants
K1 Tacrolimus
K1 Volumetric Absorptive Microsampling
K1 UniSpray
K1 LC-MS/MS
K1 Therapeutic Drug Monitoring
AB Therapeutic drug monitoring (TDM) of immunosuppressants (IMS) is crucial to prevent rejection or toxicity after solid organ transplantation. Microsampling techniques (sampling <50 μL of blood) can be a good alternative to conventional venous sampling for TDM, due to their numerous advantages, including its easy and low-invasive sampling, enabling self-collection, and cost-saving shipment and storage. Furthermore, volumetric absorptive microsampling (VAMS) enables the collection of precise and accurate blood volumes, overcoming the hematocrit (Hct) effect related to dried blood spots, while offering the same benefits. In this work, an LC-MS/MS method for the determination of the 5 most common IMS (mycophenolic acid -MPA-, tacrolimus -TAC-, sirolimus -SIR-, everolimus -EVE- and cyclosporin A -CsA-) in venous blood collected with Mitra™ VAMS devices was developed and validated, employing a novel LC-MS/MS interface, Unispray™. The method was fully validated including linearity, limits of detection (LOD) and quantification (LLOQ), accuracy, precision, selectivity, carry-over, matrix effect, recovery, impact of Hct on recovery and autosampler and short-/long-term stability, satisfying acceptance criteria in all cases. LLOQs were 0.5 ng/mL for TAC, SIR and EVE, 20 ng/mL for CsA and 75 ng/mL for MPA. No impact of the Hct (range: 0.2 to 0.62 L/L) on recovery was found for any analyte. All compounds were stable in VAMS for at least 8 months at -20 °C. In addition, as part of the VAMS analytical method validation, we performed for the first time a broad statistical study to compare liquid venous blood concentrations from patients under TAC (n = 53) and MPA (n = 20) treatment to those observed when the same specimens were absorbed into VAMS. Our results showed that venous blood VAMS concentrations were correlated to those found in the original liquid venous blood, proving that the VAMS material itself will not bias blood drug concentrations. Therefore, the present method could be applied to evaluate possible correlations between venous blood and capillary blood collected with VAMS.
PB Elsevier
SN 1873-264X
YR 2020
FD 2020
LK https://hdl.handle.net/10347/38627
UL https://hdl.handle.net/10347/38627
LA eng
NO Paniagua-González, L., Díaz-Louzao, C., Lendoiro, E., Otero-Antón, E., Cadarso-Suárez, C., López-Rivadulla, M., Cruz, A., & de-Castro-Ríos, A. (2020). Volumetric Absorptive Microsampling (VAMS) for assaying immunosuppressants from venous whole blood by LC-MS/MS using a novel atmospheric pressure ionization probe (UniSpray™). Journal of pharmaceutical and biomedical analysis, 189, 113422. https://doi.org/10.1016/j.jpba.2020.113422
DS Minerva
RD 24 abr 2026