RT Journal Article
T1 Implantable SDF-1α-loaded silk fibroin hyaluronic acid aerogel sponges as an instructive component of the glioblastoma ecosystem: Between chemoattraction and tumor shaping into resection cavities
A1 Molina-Peña, Rodolfo
A1 Ferreira, Natália Helen
A1 Roy, Charlotte
A1 Roncali, Loris
A1 Najberg, Mathie
A1 Avril, Sylvie
A1 Zarur, Mariana
A1 Bourgeois, William
A1 Ferreirós, Alba
A1 Lucchi, Chiara
A1 Cavallieri, Francesco
A1 Hindré, François
A1 Tosi, Giovani
A1 Biagini, Giuseppe
A1 Valzania, Franco
A1 Berger, François
A1 Abal, Miguel
A1 Rousseau, Audrey
A1 Boury, Frank
A1 Álvarez Lorenzo, Carmen
A1 Garcion, Emmanuel
K1 Resection cavity
K1 Implantable device
K1 Cancer cell trap
K1 Chemoattraction
K1 Ecosystem dynamic
K1 Cell homing
AB In view of inevitable recurrences despite resection, glioblastoma (GB) is still an unmet clinical need. Dealing with the stromal-cell derived factor 1-alpha (SDF-1α)/CXCR4 axis as a hallmark of infiltrative GB tumors and with the resection cavity situation, the present study described the effects and relevance of a new engineered micro-nanostructured SF-HA-Hep aerogel sponges, made of silk fibroin (SF), hyaluronic acid (HA) and heparin (Hep) and loaded with SDF-1α, to interfere with the GB ecosystem and residual GB cells, attracting and confining them in a controlled area before elimination. 70 µm-pore sponges were designed as an implantable scaffold to trap GB cells. They presented shape memory and fit brain cavities. Histological results after implantation in brain immunocompetent Fischer rats revealed that SF-HA-Hep sponges are well tolerated for more than 3 months while moderately and reversibly colonized by immuno-inflammatory cells. The use of human U87MG GB cells overexpressing the CXCR4 receptor (U87MG-CXCR4+) and responding to SDF-1α allowed demonstrating directional GB cell attraction and colonization of the device in vitro and in vivo in orthotopic resection cavities in Nude rats. Not modifying global survival, aerogel sponge implantation strongly shaped U87MG-CXCR4+ tumors in cavities in contrast to random infiltrative growth in controls. Overall, those results support the interest of SF-HA-Hep sponges as modifiers of the GB ecosystem dynamics acting as “cell meeting rooms” and biocompatible niches whose properties deserve to be considered toward the development of new clinical procedures.
PB Elsevier
SN 1742-7061
YR 2023
FD 2023
LK http://hdl.handle.net/10347/32984
UL http://hdl.handle.net/10347/32984
LA eng
NO Acta Biomaterialia Volume 173, 1 January 2024, Pages 261-282
DS Minerva
RD 23 abr 2026