RT Journal Article T1 Strategies for the valorisation of a protein-rich saline waste stream into polyhydroxyalkanoates (PHA) A1 Roibás Rozas, Alba A1 Val del Río, Ángeles A1 Hospido Quintana, Almudena A1 Mosquera Corral, Anuska K1 Bioplastics K1 High salinity wastewater K1 Industrial wastewater K1 Mixed microbial cultures K1 Protein-rich waste streams AB Saline Mussels Cooking Wastewater was valorised to produce PHA with Mixed Microbial Cultures (MMC). Due to the high protein content (1.8–5.7 g CODPROT/L), PHA accumulating capacity was below 10%, so several strategies were tested. In the acidification unit, Na(HCO3) was added, increasing protein conversion into Volatile Fatty Acids (VFA) from 10.3% to 69.2% and subsequent PHA accumulation from 6.9 to 14.7%. In the enrichment unit, the incorporation of a settling stage after the feast phase provoked a shift in the proteins’ oxidation from the feast to the famine phase, where the nitrogen released in the famine is used by the MMC for growth. This increased the biomass concentration and the tolerated COD (from 1.6 to 4.2 g VSS/L and from 2.2 to 4.38 g COD/L). Finally, varying the proteins/VFA ratio for MMC acclimation to proteins allowed increasing PHA accumulation from 8.8 to 41.5% PB Elsevier SN 0960-8524 YR 2021 FD 2021 LK http://hdl.handle.net/10347/26294 UL http://hdl.handle.net/10347/26294 LA eng NO Bioresource Technology, 334 (2021), 124964 NO This research was supported by the Spanish Government (AEI) through TREASURE [CTQ2017-83225-C2-1-R] project. The authors belong to a Galician Competitive Research Group (GRC). The programme is co-funded by the FEDER (EU) DS Minerva RD 28 abr 2026