RT Journal Article
T1 Partial Characterization of Biosurfactant from Lactobacillus pentosus and Comparison with Sodium Dodecyl Sulphate for the Bioremediation of Hydrocarbon Contaminated Soil
A1 Moldes Menduiña, Ana Belén
A1 Paradelo Núñez, Remigio
A1 Vecino Bello, Xanel
A1 Cruz Freire, José Manuel
A1 Gudiña Pérez, Eduardo José
A1 Rodrigues, Lígia Raquel Marona
A1 Teixeira, José António Couto
A1 Domínguez González, José Manuel
A1 Barral Silva, María Teresa
AB The capability of a cell bound biosurfactant produced by Lactobacillus pentosus, to accelerate the bioremediation of a hydrocarbon-contaminated soil, was compared with a synthetic anionic surfactant (sodium dodecyl sulphate SDS-). The biosurfactant produced by the bacteria was analyzed by Fourier transform infrared spectroscopy (FTIR) that clearly indicates the presence of OH and NH groups, C=O stretching of carbonyl groups and NH nebding (peptide linkage), as well as CH2–CH3 and C–O stretching, with similar FTIR spectra than other biosurfactants obtained from lactic acid bacteria. After the characterization of biosurfactant by FTIR, soil contaminated with 7,000 mg Kg−1 of octane was treated with biosurfactant from L. pentosus or SDS. Treatment of soil for 15 days with the biosurfactant produced by L. pentosus led to a 65.1% reduction in the hydrocarbon concentration, whereas SDS reduced the octane concentration to 37.2% compared with a 2.2% reduction in the soil contaminated with octane in absence of biosurfactant used as control. Besides, after 30 days of incubation soil with SDS or biosurfactant gave percentages of bioremediation around 90% in both cases. Thus, it can be concluded that biosurfactant produced by L. pentosus accelerates the bioremediation of octane-contaminated soil by improving the solubilisation of octane in the water phase of soil, achieving even better results than those reached with SDS after 15-day treatment.
PB Hindawi
SN 2314-6133
YR 2013
FD 2013
LK http://hdl.handle.net/10347/22225
UL http://hdl.handle.net/10347/22225
LA eng
NO Moldes, A. B., Paradelo, R., Vecino, X., Cruz, J. M., Gudiña, E., Rodrigues, L., ... & Barral, M. T. (2013). Partial characterization of biosurfactant from Lactobacillus pentosus and comparison with sodium dodecyl sulphate for the bioremediation of hydrocarbon contaminated soil. BioMed research international, 2013.
NO The authors wish to thank to the Spanish Ministry of Economy and Competitiveness (projects CTM 2008-01608/TECNO and CTM2012-31873)
DS Minerva
RD 30 abr 2026