RT Journal Article T1 Ultrasound-assisted extraction as a first step in a biorefinery strategy for valorisation of extracted olive pomace A1 Martínez Patiño, José Carlos A1 Gómez Cruz, Irene A1 Romero, Inmaculada A1 Gullón Estévez, Beatriz A1 Ruiz, Encarnación A1 Brnčić, Mladen A1 Castro, Eulogio K1 Extracted olive pomace K1 Response surface methodology K1 Phenolic compounds K1 Flavonoids K1 Antioxidant activity AB Currently, interest in finding new feedstock as sources of natural food antioxidants isgrowing. The extracted olive pomace (EOP), which is an agro-industrial residue from the olivepomace extracting industries, is generated yearly in big amounts, mainly in the Mediterraneancountries. EOP was subjected to an ultrasound assisted extraction with ethanol-water mixtures.The effect of main parameters, such as ethanol concentration (30–70% v/v), ultrasound amplitude(20–80%), and extraction time (5–15 min), on the extraction of antioxidant compounds was evaluatedaccording to a Box–Behnken experimental design. The antioxidant capacity of the resulting extractswas determined by measuring their content in total phenolic compounds (TPC) and flavonoids (TFC),as well as their antioxidant activity by DPPH, ferric reducing antioxidant power (FRAP), and ABTSassays. Considering the simultaneous maximization of these five responses, the optimal conditionswere found to be 43.2% ethanol concentration, 70% amplitude, and 15 min. The ultrasound assistedextraction of EOP under these optimized conditions yielded an extract with a phenolic and flavonoidcontent (per gram of EOP) of 57.5 mg gallic acid equivalent (GAE) and 126.9 mg rutin equivalent(RE), respectively. Likewise, the values for DPPH, ABTS, and FRAP assay (per gram of EOP) of 56.7,139.1, and 64.9 mg Trolox equivalent, respectively were determined in the optimized extract PB MDPI YR 2019 FD 2019 LK http://hdl.handle.net/10347/22195 UL http://hdl.handle.net/10347/22195 LA eng NO Martínez-Patiño, J.C., Gómez-Cruz, I., Romero, I., Gullón, B., Ruiz, E., Brnčić, M., Castro, E. (2019). Ultrasound-assisted extraction as a first step in a biorefinery strategy for valorisation of extracted olive pomace. Energies 12(14), 2679. doi: 10.3390/en12142679 NO This research was funded by the Spanish Ministerio de Economía y Competitividad- Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional. Project Reference ENE2017-85819-C2-1-R DS Minerva RD 24 abr 2026