RT Journal Article
T1 Valorization of bioethanol by-products to produce unspecific peroxygenase with Agrocybe aegerita: Technological and proteomic perspectives
A1 González Rodríguez, Sandra
A1 Trueba Santiso, Alba María
A1 Lu Chau, Thelmo Alejandro
A1 Moreira Vilar, María Teresa
A1 Eibes González, Gemma María
K1 Unspecific peroxygenase (UPO)
K1 Agrocybe aegerita
K1 Vinasse
K1 Submerged fermentation
K1 Residues valorization
K1 Proteomic analysis
AB Unspecific peroxygenase (UPO) presents a wide range of biotechnological applications. This study targets the use of by-products from bioethanol synthesis to produce UPO by Agrocybe aegerita. Solid-state and submerged fermentations (SSF and SmF) were evaluated, achieving the highest titers of UPO and laccase in SmF using vinasse as nutrients source. Optimized UPO production of 331 U/L was achieved in 50% (v:v) vinasse with an inoculum grown for 14 days. These conditions were scaled-up to a 4 L reactor, achieving a UPO activity of 265 U/L. Fungal proteome expression was analyzed before and after UPO activity appeared by shotgun mass spectrometry proteomics. Laccase, dye-decolorizing peroxidases (DyP), lectins and proteins involved in reactive oxygen species (ROS) production and control were detected (in addition to UPO). Interestingly, the metabolism of complex sugars and nitrogen sources had a different activity at the beginning and end of the submerged fermentation
PB Elsevier
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30658
UL http://hdl.handle.net/10347/30658
LA eng
NO New BIOTECHNOLOGY 76 (2023) 63–71
NO S.G., A.T. and G.E. thank their grants (BES-2017-081677, FJC2019-041664-I and RYC2018-024846-I, respectively) funded by MCIN/AEI/ 10.13039/501100011033, and by “ERDF A way of making Europe” and “ESF Investing in your future”. Authors would like to thank the use of USC Mass Spectrometry and Proteomics facilities and Bioetanol Galicia S.A. for the supply of the substrates used in the fermentations. The authors belong to the Galician Competitive Research Groups (GRC)_ ED431C-2021/37. The program is co-funded by FEDER (UE)
DS Minerva
RD 26 abr 2026