RT Journal Article
T1 Differential behaviour of epicardial adipose tissue-secretomes with high and low orosomucoid levels from patients with cardiovascular disease in H9C2 cells
A1 Moscoso, Isabel
A1 Fernandez-Trasancos, Ángel
A1 Cabro, María
A1 Couselo, Marinela
A1 Fandiño-Vaquero, Rubén
A1 Bravo, Susana B.
A1 Sierra, Juan
A1 Eiras, Sonia
A1 Lage Fernández, Ricardo
A1 González Juanatey, José Ramón
K1 Epicardial fat
K1 Secretome
K1 Orosomucoid
K1 H9C2 cells
AB Epicardial adipose tissue releases orosomucoid (ORM), an acute phase protein with multiple modulatory and protective properties. We aimed to identify the effect of EAT-supernatants according to their ORM levels on H9C2 cells. H9C2 were cultured with EAT-secretomes or ORM protein itself on a Real-Time Cell Analyser. Secretome proteins identification was performed by LC-mass spectrometry according to their ORM levels. Two of them were validated by ELISA in EAT-supernatants from 42 patients. ORM effect on H9C2 and neonatal rat cardiomyocytes apoptosis under hypoxia with or without fatty acid treatment was determined by Annexin-V flow cytometry measurement. Caspase-3 expression levels were determined by western blot in H9C2. Our results showed a differential effect of EAT-secretomes according their ORM levels. Although additional secreted proteins can contribute to their beneficial effects, ORM reduced hypoxia-induced apoptosis through caspase-3 inhibition. Our data showed the cardioprotective role of ORM and suggest that its quantification on EAT secretomes might help us to find new secreted factors with a cardioprotective role.
PB Elsevier
SN 0303-7207
YR 2015
FD 2015-11-15
LK https://hdl.handle.net/10347/38872
UL https://hdl.handle.net/10347/38872
LA eng
NO Ricardo Lage, Isabel Moscoso, Ángel Fernández-Trasancos, María Cebro, Marinela Couselo, Rubén Fandiño-Vaquero, Susana B. Bravo, Juan Sierra, José Ramón González-Juanatey, Sonia Eiras, Differential behaviour of epicardial adipose tissue-secretomes with high and low orosomucoid levels from patients with cardiovascular disease in H9C2 cells, Molecular and Cellular Endocrinology, Volume 416, 2015, Pages 77-87, ISSN 0303-7207, https://doi.org/10.1016/j.mce.2015.08.025.
DS Minerva
RD 25 abr 2026