RT Journal Article
T1 Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis
A1 Martínez Sernández, Victoria
A1 Muiño Pose, Laura
A1 Perteguer, María Jesús
A1 Gárate, Teresa
A1 Mezo, Mercedes
A1 González Warleta, Marta
A1 Muro, Antonio
A1 Costa, José Manuel Correia da
A1 Romarís Martínez, Fernanda
A1 Martínez Ubeira, Florencio César
AB Background: Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F.hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katztest, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate fordetection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunologicalmethods may be a good alternative and are recommended for use in major hospitals where trained personnel are available,although they are not usually implemented for individual testing.Methodology/Principal Findings: We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of humanfasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAbMM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) theSeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples.Conclusions/Significance: The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required.
PB PLOS
SN 1935-2727
YR 2011
FD 2011
LK http://hdl.handle.net/10347/22036
UL http://hdl.handle.net/10347/22036
LA eng
NO Martínez-Sernández V., Muíño L., Perteguer M.J., Gárate T., Mezo M., et al. (2011). Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis. PLoS Negl Trop Dis 5(11): e1376. https://doi.org/10.1371/journal.pntd.0001376
NO The present study was supported by grants AGL2006-13936-C02 and AGL2010-22290-C03 (Ministerio de Ciencia e Innovación, Spain), grant 2010–13(Fundación Ramón Areces, Spain), and by the European Fund for Regional Development (FEDER). Laura Muiño is supported by grant INCITE09 PXIB 203 206 PR(Xunta de Galicia, Spain)
DS Minerva
RD 1 may 2026