RT Dissertation/Thesis
T1 New pulmonary delivery platform based on chitosan nanocapsules with potential application in gene therapy
A1 Fernández Paz, Estefanía
K1 Chitosan nanocapsules
K1 dry powders
K1 microencapsulation
K1 pCMV-betaGal
K1 pulmonary gene delivery
K1 spray-drying
AB Gene therapy offers hope of cure for rare and other serious diseases that currently have no established effective treatments. It has become a valuable therapeutic strategy for congenital or acquired genetic lung diseases, such as alpha1-antitrypsin deficiency, infections and some types of lung cancer, among others. In this Thesis work, a new pulmonary delivery platform consisting of mannitol microspheres (Ma MS) containing chitosan-based nanocapsules (CS-based NCs) loaded with the model plasmid pCMV-betaGal is proposed for its direct delivery to the lung. The designed micro-nanostructures have suitable aerodynamic properties, are biocompatible with the A549 lung cell line and are distributed in the deep lung, producing transfection.
YR 2022
FD 2022
LK http://hdl.handle.net/10347/28134
UL http://hdl.handle.net/10347/28134
LA eng
DS Minerva
RD 28 abr 2026