RT Journal Article
T1 Preclinical characterization of endotoxin-induced uveitis models using OCT, PET/CT and proteomics
A1 Cuartero Martínez, Andrea
A1 García Otero, Xurxo
A1 Codesido, Jessica
A1 Gómez Lado, Noemí
A1 Mateos, Jesús
A1 Bravo, Susana B.
A1 Rodríguez-Fernández, Carmen Antía
A1 González Barcia, Miguel
A1 Aguiar Fernández, Pablo
A1 Ortega-Hortas, Marcos
A1 Otero Espinar, Francisco Javier
A1 Fernández Ferreiro, Anxo
K1 Model characterization
K1 Endotoxin-induced uveitis models
K1 Ocular inflammatory diseases
K1 OCT imaging
K1 Proteomics
K1 PET/CT imaging
AB Uveitis is a group of inflammatory ocular pathologies. Endotoxin-Induced Uveitis (EIU) model represent a well-known model induced by administration of Lipopolysaccharide (LPS). The aim is to characterize two models of EIU through two routes of administration with novel noninvasive imaging techniques. 29 rats underwent Intraocular Pressure (IOP) measurements, Optical Coherence Tomography (OCT), proteomic analysis, and Positron Emission Tomography and Computed Tomography (PET/CT). Groups included healthy controls (C), BSS administered controls (Ci), systemically induced EIU with LPS (LPSs), and intravitreally induced EIU with LPS (LPSi) for IOP, OCT, and proteomic studies. For 18F-FDG PET/CT study, animals were divided into FDG-C, FDG-LPSs and FDG-LPSi groups and scanned using a preclinical PET/CT system. LPSi animals exhibited higher IOP post-induction compared to C and LPSs groups. LPSi showed increased cellular infiltrate, fibrotic membranes, and iris inflammation. Proinflammatory proteins were more expressed in EIU models, especially LPSi. PET/CT indicated higher eye uptake in induced models compared to FDG-C. FDG-LPSi showed higher eye uptake than FDG-LPSs but systemic uptake was higher in FDG-LPSs due to generalized inflammation. OCT is valuable for anterior segment assessment in experimental models. 18F-FDG PET/CT shows promise as a noninvasive biomarker for ocular inflammatory diseases. Intravitreal induction leads to higher ocular inflammation. These findings offer insights for future inflammatory disease research and drug studies.
PB Elsevier
SN 0378-5173
YR 2024
FD 2024-07-25
LK https://hdl.handle.net/10347/39754
UL https://hdl.handle.net/10347/39754
LA eng
NO References  Cuartero-Martínez, A., García-Otero, X., Codesido, J., Gómez-Lado, N., Mateos, J., Bravo, S. B., Rodríguez-Fernández, C. A., González-Barcia, M., Aguiar, P., Ortega-Hortas, M., Otero-Espinar, F. J., & Fernández-Ferreiro, A. (2024). Preclinical characterization of endotoxin-induced uveitis models using OCT, PET/CT and proteomics. International Journal of Pharmaceutics, 66210.1016/j.ijpharm.2024.124516
NO This work was partially supported by Instituto de Salud Carlos III (ISCIII, Spain) through PI20/00719 project, Axencia Galega de Innovación (Grupos de Potencial Crecimiento IN607B2020/11, Spain), Ministry of Science and Innovation of Spain (MICINN, Spain) through PID2022-142350OB-C21 project and Xunta de Galicia (Grupo de Referencia Competitiva, ED431C 2021/26, Spain). X. García-Otero and J. Mateos acknowledges the support of Xunta de Galicia (Spain) through the postdoctoral fellowship [ED481B-2023-063] and Senior Talent Research grant [11-IN858A_2021_1141142]. J. Codesido is grateful to the Health Research Institute of Santiago de Compostela (IDIS, Spain) for financing his predoctoral research fellowship.
DS Minerva
RD 25 abr 2026