RT Journal Article
T1 Machine Learning Technology Reveals the Concealed Interactions of Phytohormones on Medicinal Plant In Vitro Organogenesis
A1 García Pérez, Pascual
A1 Lozano Milo, Eva
A1 Landín Pérez, Mariana
A1 Gallego, Pedro Pablo
K1 Algorithms
K1 Artificial intelligence
K1 Auxins
K1 Cytokinins
K1 In vitro culture
K1 Kalanchoe
K1 Plant growth regulators (PGRs)
K1 Plant tissue culture
AB Organogenesis constitutes the biological feature driving plant in vitro regeneration, in which the role of plant hormones is crucial. The use of machine learning (ML) technology stands out as a novel approach to characterize the combined role of two phytohormones, the auxin indoleacetic acid (IAA) and the cytokinin 6-benzylaminopurine (BAP), on the in vitro organogenesis of unexploited medicinal plants from the Bryophyllum subgenus. The predictive model generated by neurofuzzy logic, a combination of artificial neural networks (ANNs) and fuzzy logic algorithms, was able to reveal the critical factors affecting such multifactorial process over the experimental dataset collected. The rules obtained along with the model allowed to decipher that BAP had a pleiotropic effect on the Bryophyllum spp., as it caused different organogenetic responses depending on its concentration and the genotype, including direct and indirect shoot organogenesis and callus formation. On the contrary, IAA showed an inhibiting role, restricted to indirect shoot regeneration. In this work, neurofuzzy logic emerged as a cutting-edge method to characterize the mechanism of action of two phytohormones, leading to the optimization of plant tissue culture protocols with high large-scale biotechnological applicability
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23663
UL http://hdl.handle.net/10347/23663
LA eng
NO García-Pérez, P.; Lozano-Milo, E.; Landín, M.; Gallego, P.P. Machine Learning Technology Reveals the Concealed Interactions of Phytohormones on Medicinal Plant In Vitro Organogenesis. Biomolecules 2020, 10, 746
NO The authors acknowledge the FPU grant awarded to Pascual García-Pérez from the Spanish Ministry of Education (grant number FPU15/04849)
DS Minerva
RD 27 abr 2026