RT Journal Article
T1 Microsatellite markers for the molecular characterisation of Neospora caninum: application to clinical samples
A1 Pedraza-Díaz, Susana
A1 Marugán Hernández, Virginia
A1 Collantes Fernández, Esther
A1 Regidor Cerrillo, Javier
A1 Rojo Montejo, Silvia
A1 Gómez Bautista, Mercedes
A1 Ortega Mora, Luis Miguel
K1 Neospora caninum
K1 Bovine aborted foetuses
K1 Microsatellite markers
K1 Nested PCR
AB We report here on the development of a nested PCR procedure for the application of Neospora caninum microsatellite markers to clinical samples. Genotyping technology by fluorescently labelled DNA fragment analysis was used in combination with DNAsequencing for those markers which show additional SNPs or complex repetitive sequences. Twenty-nine DNA samples from naturally infected bovine aborted foetuses from two regions of Spain where N. caninum had been detected by nested PCR of the ITS1rRNA region were analysed using these microsatellites. Complete, or almost complete allele profiles were obtained from 18 samples. Two pairs of DNA samples showed identical profiles, these originated from twins and foetuses from the same herd, respectively. The multilocus analysis performed showed sub clustering of isolates according to their geographical origin. These results highlight the usefulness of these markers for the molecular characterization of isolates of N. caninum and for isolate tracking in live vaccine development.
PB Elsevier
YR 2009
FD 2009
LK https://hdl.handle.net/10347/39495
UL https://hdl.handle.net/10347/39495
LA eng
NO Veterinary Parasitology Volume 166, Issues 1–2, 3 December 2009, Pages 38-46
NO This work was partially funded by I.N.I.A. (RTA04-047-C2) and the Spanish Ministry of Education and Science (AGL2007-60132/GAN). V. Marugán-Hernández and J. Regidor-Cerrillo were funded by the Spanish Ministry of Education and Science and by Consejería de Educación (Comunidad de Madrid), respectively. This work was part of the E.U. research collaboration COST Action-857.
DS Minerva
RD 26 abr 2026