RT Journal Article
T1 Implantable controlled release devices for BMP-7 delivery and suppression of glioblastoma initiating cells
A1 Reguera-Nuñez, Elaine
A1 Roca, Carlota
A1 Hardy, Eugenio
A1 Fuente, María de la
A1 Csaba, Noemi Stefania
A1 García-Fuentes, Marcos
K1 Cancer initiating cells
K1 Glioblastoma
K1 Controlled release
K1 Bone morphogenetic protein
K1 Microspheres
K1 PLGA
AB Designing therapeutic devices capable of manipulating glioblastoma initiating cells (GICs) is critical to stop tumor recurrence and its associated mortality. Previous studies have indicated that bone morphogenetic protein-7 (BMP-7) acts as an endogenous suppressor of GICs, and thus, it could become a treatment for this cancer. In this work, we engineer an implantable microsphere system optimized for the controlled release of BMP-7 as a bioinspired therapeutic device against GICs. This microsphere delivery system is based on the formation of a heparin- BMP-7 nanocomplex, first coated with Tetronic® and further entrapped in a biodegradablepolyester matrix. The obtained microspheres can efficiently encapsulate BMP-7, and release itin a controlled manner with minimum burst effect for over two months while maintaining protein bioactivity. Released BMP-7 showed a remarkable capacity to stop tumor formation in a GICs cell culture model, an effect that could be mediated by forced reprogramming of tumorigenic cells towards a non-tumorigenic astroglial lineage.
PB Elsevier
SN 0142-9612
YR 2014
FD 2014-01-07
LK http://hdl.handle.net/10347/9865
UL http://hdl.handle.net/10347/9865
LA eng
NO Reguera-Nuñez et al. Biomaterials 35, pp. 2859-2867 (2014)
NO This work was financed by Ministerio de Ciencia e Innovación, Programa de Investigación en Salud (PS09/1786) and Xunta de Galicia (EM2013/042). ERN acknowledges a grant from Agencia Española de Cooperación Internacional. MF had a contract from Asociación Española contra el Cáncer. NC and MGF acknowledge their Isidro Parga Pondal contracts from Xunta de Galicia. Dr. Helena Mira and Dr. Pilar González advised us on the neurosphere culture model and the cell testing assays. Katrin Viertel and Ida Fejos provided technical help at the preformulation stages. Prof. Anxo Vidal and Erea Borrajo provided technical help for FACS analysis. We thank Prof. Maria J. Alonso for the supporting group infrastructure.
DS Minerva
RD 24 abr 2026