RT Journal Article
T1 Isothermal microcalorimetry for scaffold design and characterization: Assessing bacterial and host cell interactions and physicochemical stability
A1 Álvarez Lorenzo, Carmen
A1 Concheiro Nine, Ángel Joaquín
K1 Isothermal microcalorimetry
K1 Interface events
K1 Tissue engineering
K1 Infection
K1 Biofilm
K1 Stability
K1 Cell growth
AB Scaffolds used in regenerative medicine are increasingly expected to address personalization, bioactivity, and sustainability, underscoring the need for characterization methods that reliably predict safety and efficacy. Isothermal microcalorimetry (IMC) offers a highly sensitive, label-free, real-time measurement of heat flow from energy-generating or -consuming process at scaffold interfaces. By monitoring microbial activity, host cell metabolism, material stability, and responses to environmental or therapeutic factors, IMC provides physiologically relevant insight into scaffold performance over extended periods. Its non-destructive, low-preparation, and passive nature preserves samples for complementary analyses, making it a versatile yet underutilized tool in biomedical research. This review introduces IMC for scaffold design and characterization, emphasizing its capacity to evaluate vulnerability to biofilm formation and the effectiveness of anti-biofilm strategies. It further explores applications in tracking scaffold formation, assessing host cell-material interactions and tissue development, and probing the antitumor potential of engineered scaffolds. The review concludes with a perspective on IMC's role in advancing scaffold translation within the evolving regulatory landscape shaped by the FDA Modernization Acts 2.0 and 3.0.
PB Elsevier
SN 0001-8686
YR 2025
FD 2025-12
LK https://hdl.handle.net/10347/43883
UL https://hdl.handle.net/10347/43883
LA eng
NO Alvarez-Lorenzo, C., & Concheiro, A. (2025). Isothermal microcalorimetry for scaffold design and characterization: Assessing bacterial and host cell interactions and physicochemical stability. Advances in Colloid and Interface Science, 346, 103681. 10.1016/j.cis.2025.103681
NO The work was supported by Spain Ministerio de Ciencia, Innovación y Universidades MICIU/AEI/ 10.13039/501100011033 [PID2023- 150422OB-I00], ERDF A way of making Europe, cofunded by the Eu- ropean Union, and Xunta de Galicia [ED431C 2024/09].
DS Minerva
RD 24 abr 2026