RT Journal Article
T1 Tracking Bacterial Spoilage in Cosmetics by a New Bioanalytical Approach: API-SPME-GC-MS to Monitor MVOCs
A1 Celeiro Montero, María
A1 Varela, Esther
A1 Rodríguez, Rocío
A1 Penedo, Manuel
A1 Lores Aguín, Marta
K1 Microbial volatile organic compounds
K1 Bacterial contamination
K1 Consumers’ health
K1 Analytical profile index
K1 Solid-phase microextraction
K1 Gas chromatography-mass spectrometry
AB The main goal of this work was the use of the powerful solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) technique to unequivocally identify microbial volatile organic compounds (MVOCs) derived from the enzymatic activity produced during metabolic processes using analytical profile index (API) biochemical tests. Three bacteria were selected for this study: Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. They were inoculated and incubated to both API components and real cosmetics, as well as to a mixture of them. Specific MVOCs were successfully identified as biomarkers for each one of the studied microorganisms: Indole and 2-nitrophenol as Escherichia coli markers, 2-undecanone and phenylethyl alcohol as Proteus mirabilis-specific markers, and 1-undecene and 2′-aminoacetophenone as Pseudomonas aeruginosa ones. In addition, a high number of MVOCs were identified as general markers of bacterial presence. The results revealed that the MVOCs’ formation is highly subtract dependent. Therefore, the ultimate and most challenging objective is to establish a relationship between the identified MVOCs and the original compound present in the substrate. This work establishes the design and development of this original approach, and its practical application to the control of microbial contamination in real cosmetic samples
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23704
UL http://hdl.handle.net/10347/23704
LA eng
NO Celeiro, M.; Varela, E.; Rodriguez, R.; Penedo, M.; Lores, M. Tracking Bacterial Spoilage in Cosmetics by a New Bioanalytical Approach: API-SPME-GC-MS to Monitor MVOCs. Cosmetics 2020, 7, 38
NO This research was funded by project QFashion (IN852A 2016/157, CONECTA-PEME, Xunta de Galicia). The authors belong to the CRETUS Strategic Partnership (ED431E 2018/01). All these programmes are co-funded by FEDER (UE)
DS Minerva
RD 28 abr 2026