RT Journal Article
T1 Detection of mycotoxins in cheese using an optimized analytical method based on a QuEChERS extraction and UHPLC-MS/MS quantification
A1 Rodríguez Cañás, Inés
A1 González Jartín, Jesús María
A1 Alvariño Romero, Rebeca
A1 Alfonso Rancaño, María Amparo
A1 Rodríguez Vieytes, Mercedes
A1 Botana López, Luis Miguel
K1 Mycotoxin
K1 Cheese
K1 UHPLC
K1 Mass spectrometry
K1 QuEChERS
AB Mycotoxins can produce toxic effects on humans; hence, it is of high importance to determine their presence in food products. This work presents a reliable method for the quantification of 32 mycotoxins in cheese. The analysis procedure was optimized based on a QuEChERS extraction process and the ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) detection. The analysis method was validated for four cheese varieties (emmental, blue, brie and camembert) in terms of linearity, sensitivity, matrix effect, accuracy and precision. Satisfactory precision and accuracy values were achieved, with recoveries above 70% for most mycotoxins. The developed method was applied to the analysis of 38 commercial cheese samples. A high occurrence of beauvericin and enniatins were found, ranging from 31% for enniatin A to 100% for enniatin B. The ochratoxin A was detected in three samples at concentrations that may pose a risk to human health
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29901
UL http://hdl.handle.net/10347/29901
LA eng
NO Food Chemistry 408 (2023) 135182
NO The research leading to these results has received funding from the following FEDER cofunded-grants. From Conselleria de Cultura, Educacion e Ordenación Universitaria, Xunta de Galicia, GRC (ED431C 2021/01). From Ministerio de Ciencia e Innovación IISCIII/PI19/001248, PID 2020-11262RB-C21. From European Union Interreg Agritox EAPA-998-2018, and H2020 778069-EMERTOX. R. A. is supported by a postdoctoral fellowship from Xunta de Galicia (ED481B-2021-038), Spain
DS Minerva
RD 4 may 2026