RT Journal Article
T1 Development of optokinetic tracking software for objective evaluation of visual function in rodents
A1 Segura, Francisco
A1 Arines Piferrer, Justo
A1 Sánchez Cano, Ana
A1 Perdices, Lorena
A1 Orduna Hospital, Elvira
A1 Fuentes Broto, Lorena
A1 Pinilla, Isabel
K1 Smooth pursuit
K1 Vestibuloocular reflex
AB The aim of this study was to develop software that performs the optokinetic tracking assessment without the involvement of experimenters to increase the objectivity of the test. To check the effectiveness of the software, several videos were analyzed and the results were compared to those produced by two experimenters. Videos consisted of visual acuity and contrast sensitivity tests on normal animals and pigmented P23H rats (animal model of retinitis pigmentosa). Our software showed a reasonably high success rate: in approximately 78% of the cases, both the software program and the experimenters were in agreement, including the direction of rotation. The software detected 7% false positive cases, 10% false negative cases, and it was wrong in 5% of the cases. Decrease in visual function with age in pigmented P23H rats was observed from the first time interval, although minimum thresholds were found in visual parameters at advanced ages. We developed simple software based on current functions included in the Matlab image processing toolbox that was able to recognize, with a reasonably high percentage of success, the subtle head movements of the rodent produced when visual perception of the optokinetic optotype occurs
PB Springer Nature
YR 2018
FD 2018-07-03
LK http://hdl.handle.net/10347/17659
UL http://hdl.handle.net/10347/17659
LA eng
NO Segura, F., Arines, J., Sánchez-Cano, A., Perdices, L., Orduna-Hospital, E., Fuentes-Broto, L., & Pinilla, I. (2018). Development of optokinetic tracking software for objective evaluation of visual function in rodents. Scientific Reports, 8(1). doi: 10.1038/s41598-018-28394-x
NO Publication of this article was supported by the General Council of Aragon (Diputación General de Aragón) Group B99, Health Research Fund Instituto de Salud Carlos III (Fondo de Investigación Sanitaria, Spanish Ministry of Health) PI13/01124, and Health Institute Carlos III (Instituto de Salud Carlos III) RETICS RD16/0008/0016, ED431B 2017/64 (Xunta de Galicia and FEDER), and FIS2016–77319 (Ministerio de Industria, Economía y Competitividad and FEDER)
DS Minerva
RD 24 abr 2026