RT Journal Article
T1 RNA-Seq Transcriptome Profiling of the Queen Scallop (Aequipecten opercularis) Digestive Gland after Exposure to Domoic Acid-Producing Pseudo-nitzschia
A1 Ventoso Osorio, Pablo
A1 Pazos Castelos, Antonio Juan
A1 Pérez-Parallé Mera, María Luz
A1 Blanco Pérez, Juan Carlos
A1 Triviño, Juan Carlos
A1 Sánchez López, José Luis
K1 Amnesic shellfish poisoning (ASP)
K1 Domoic acid
K1 Bivalves
K1 Aequipecten opercularis
K1 Scallop
K1 RNA-seq
K1 Transcriptome
K1 Differential expression
K1 QPCR
K1 Oxidative stress
AB Some species of the genus Pseudo-nitzschia produce the toxin domoic acid, which causes amnesic shellfish poisoning (ASP). Given that bivalve mollusks are filter feeders, they can accumulate these toxins in their tissues. To elucidate the transcriptional response of the queen scallop Aequipecten opercularis after exposure to domoic acid-producing Pseudo-nitzschia, the digestive gland transcriptome was de novo assembled using an Illumina HiSeq 2000 platform. Then, a differential gene expression analysis was performed. After the assembly, 142,137 unigenes were obtained, and a total of 10,144 genes were differentially expressed in the groups exposed to the toxin. Functional enrichment analysis found that 374 Pfam (protein families database) domains were significantly enriched. The C1q domain, the C-type lectin, the major facilitator superfamily, the immunoglobulin domain, and the cytochrome P450 were among the most enriched Pfam domains. Protein network analysis showed a small number of highly connected nodes involved in specific functions: proteasome components, mitochondrial ribosomal proteins, protein translocases of mitochondrial membranes, cytochromes P450, and glutathione S-transferases. The results suggest that exposure to domoic acid-producing organisms causes oxidative stress and mitochondrial dysfunction. The transcriptional response counteracts these effects with the up-regulation of genes coding for some mitochondrial proteins, proteasome components, and antioxidant enzymes (glutathione S-transferases, thioredoxins, glutaredoxins, and copper/zinc superoxide dismutases)
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21666
UL http://hdl.handle.net/10347/21666
LA eng
NO Ventoso, P., Pazos, A. J., Pérez-Parallé, M. L., Blanco, J., Triviño, J. C., & Sánchez, J. L. (2019). Aequipecten opercularis) Digestive Gland after Exposure to Domoic Acid-Producing Pseudo-nitzschia. Toxins, 11(2), 97. MDPI AG. Retrieved from http://dx.doi.org/10.3390/toxins11020097
NO This work has been supported by the Spanish Ministry MINECO (Ministerio de Economía y Competitividad) and the FEDER Funds (European Regional Development Fund) of the European Union under the project AGL2012-39972-C02
DS Minerva
RD 24 abr 2026