RT Journal Article
T1 Real-Time Evaluation of Binding Mechanisms in Multivalent Interactions: A Surface Plasmon Resonance Kinetic Approach
A1 Munoz, Eva Maria
A1 Correa, Juan
A1 Riguera Vega, Ricardo
A1 Fernández Megía, Eduardo
AB Multivalency is a key, ubiquitous phenomenon in nature characterized by a complex combination of binding mechanisms, with special relevance in carbohydrate–lectin recognition. Herein we introduce an original surface plasmon resonance kinetic approach to analyze multivalent interactions that has been validated with dendrimers as monodisperse multivalent analytes binding to lectin clusters. The method, based on the analysis of early association and late dissociation phases of the sensorgrams provides robust information of the glycoconjugate binding efficiency and real-time structural data of the binding events under the complex scenario of the glyco-cluster effect. Notably, it reveals the dynamic nature of the interaction and offers experimental evidence on the contribution of binding mechanisms
PB American Chemical Society
SN 0002-7863
YR 2013
FD 2013
LK http://hdl.handle.net/10347/16954
UL http://hdl.handle.net/10347/16954
LA eng
NO Munoz, E., Correa, J., Riguera, R., & Fernandez-Megia, E. (2013). Real-Time Evaluation of Binding Mechanisms in Multivalent Interactions: A Surface Plasmon Resonance Kinetic Approach. Journal Of The American Chemical Society, 135(16), 5966-5969. doi: 10.1021/ja400951g
NO This work was financially supported by the Spanish Government (CTQ2009-10963, CTQ2012-34790, CTQ2009-14146-C02-02) and the Xunta de Galicia (10CSA209021PR and CN2011/037). E.M.M. thanks the Xunta de Galicia for a “Parga Pondal” research contract
DS Minerva
RD 23 abr 2026