RT Journal Article
T1 Forensic evaluation of the Asia Pacific ancestry-informative MAPlex assay
A1 Xavier, Catarina
A1 Puente Vila, María del Carmen de la
A1 Phillips, Christopher Paul
A1 Eduardoff, Mayra
A1 Heidegger, A.
A1 Mosquera Miguel, Ana
A1 Freire Aradas, Ana María
A1 Lagacé, Robert E.
A1 Wootton, Sharon Chao
A1 Power, Daniel
A1 Parson, Walther
A1 Lareu Huidobro, María Victoria
A1 Daniel, Runa
K1 Biogeographical ancestry estimation
K1 Asia-Pacific populations
K1 Massive parallel sequencing
K1 Ion S5 sequencing
AB DNA intelligence, and particularly the inference of biogeographical ancestry (BGA) is increasing in interest, and relevance within the forensic genetics community. The majority of current MPS-based forensic ancestry-informative assays focus on the differentiation of major global populations. The recently published MAPlex (Multiplex for the Asia Pacific) panel contains 144 SNPs and 20 microhaplotypes and aims to improve the differentiation of populations in the Asia Pacific region. This study reports the first forensic evaluation of the MAPlex panel using AmpliSeq technology and Ion S5 sequencing. This study reports on the overall performance of MAPlex including the assay’s sequence coverage distribution and stability, baseline noise and description of problematic SNPs. Dilution series, artificially degraded and mixed DNA samples were also analysed to evaluate the sensitivity of the panel with challenging or compromised forensic samples. As the first panel to combine biallelic SNPs, multiple-allele SNPs and microhaplotypes, the MAPlex assay demonstrated an enhanced capacity for mixture detection, not easily performed with common binary SNPs. This performance evaluation indicates that MAPlex is a robust, stable and highly sensitive assay that is applicable to forensic casework for the prediction of BGA
PB Elsevier
SN 1872-4973
YR 2020
FD 2020
LK http://hdl.handle.net/10347/24242
UL http://hdl.handle.net/10347/24242
LA eng
NO Forensic Science International: Genetics, Volume 48, September 2020, 102344
NO MdlP is supported by a postdoctoral fellowship awarded by the Consellería de Cultura, Educación e Ordenación Universitaria and the Consellería de Economía, Emprego e Industria from Xunta de Galicia (Modalidade A, ED481B 2017/088). CP, AFA, AMM, MdlP, MVL are supported by MAPA, Multiple Allele Polymorphism Analysis (BIO2016-78525-R), a research project funded by the Spanish Research State Agency (AEI), and co-financed with ERDF funds. AFA is supported by a post-doctorate grant funded by the Consellería de Cultura, Educación e Ordenación Universitaria e da Consellería de Economía, Emprego e Industria from Xunta de Galicia, Spain (Modalidade B, ED481B 2018/010). The 1000 Genomes high coverage sequence data were generated at the New York Genome Center with funds provided by NHGRI Grant 3UM1HG008901-03S1
DS Minerva
RD 24 abr 2026