RT Journal Article
T1 Comparative expression analysis in mature gonads, liver and brain of turbot (Scophthalmus maximus) by cDNA-AFLPS
A1 Taboada Penoucos, Xoana
A1 Robledo Sánchez, Diego
A1 Palacio Rodríguez, Lorena del
A1 Rodeiro, Antonio
A1 Felip, Alicia
A1 Martínez Portela, Paulino
A1 Viñas Díaz, Ana María
K1 DNA markers
K1 Fishes
K1 Sex differentiation
K1 Transcriptome
K1 Sexual dimorphism
AB Turbot is one of the most important farmed fish in Europe. This species exhibits a considerable sexual dimorphism in growth and sexual maturity that makes the all-female production recommended for turbot farming. Our knowledge about the genetic basis of sex determination and the molecular regulation of gonad differentiation in this species is still limited. Our goal was to identify and compare gene expression and functions between testes and ovaries in adults in order to ascertain the relationship between the genes that could be involved in the gonad differentiation or related to the sex determination system. The identification of differentially expressed sex related genes is an initial step towards understanding the molecular mechanisms of gonad differentiation. For this, we carried out a transcriptome analysis based on cDNA-AFLP technique which allowed us to obtain an initial frame on sex-specific gene expression that will facilitate further analysis especially along the critical gonad differentiating period. With the aim of widening the study on sex-biased gene expression we reproduced the same experiments in two somatic tissues: liver and brain. We have selected the liver because it is the most analyzed one regarding sexual dimorphic gene expression and due to its importance in steroid hormones metabolism and the brain because the functional relationship between brain and gonad is documented. We found slight but important differences between sexes which deserve further investigation
PB Elsevier
SN 0378-1119
YR 2016
FD 2016
LK http://hdl.handle.net/10347/26151
UL http://hdl.handle.net/10347/26151
LA eng
NO Gene 492(1): 250-261 (2012)
NO This research work was financially supported by the Xunta of Galicia (07MMA004200PR) to A. Viñas. X. Taboada was supported by a fellowship from the European Social Fund and Consellería de Educación e Ordenación Universitaria- Xunta de Galicia (Spain)
DS Minerva
RD 30 abr 2026