RT Journal Article
T1 Microfluidics-Driven Manufacturing and Multiscale Analytical Characterization of Nanoparticle-Vesicle Hybrids
A1 Cardellini, Jacopo
A1 González Gómez, Manuel Antonio
A1 Rivas Rey, José
A1 Arosio, Paolo
K1 Acoustofluidics
K1 Lipid vesicles
K1 Microfluidics
K1 Nanoparticle-vesicle hybrids
K1 Nanoparticles
AB Coating synthetic nanoparticles (NPs) with lipid membranes is a promising approach to enhance the performance of nanomaterials in various biological applications, including therapeutic delivery to target organs. Current methods for achieving this coating often rely on bulk approaches which can result in low efficiency and poor reproducibility. Continuous processes coupled with quality control represent an attractive strategy to manufacture products with consistent attributes and high yields. Here, this concept is implemented by developing an acoustic microfluidic device together with an analytical platform to prepare nanoparticle-vesicle hybrids and quantitatively characterize the nanoparticle coverage using fluorescence-based techniques at different levels of resolution. With this approach polymethyl methacrylate (PMMA) nanoparticles are successfully coated with liposomes and extracellular vesicles (EVs), achieving a high encapsulation efficiency of 70%. Moreover, the approach enables the identification of design rules to control the efficiency of encapsulation by tuning various operational parameters and material properties, including buffer composition, nanoparticle/vesicle ratio, and vesicle rigidity.
PB Wiley
SN 2192-2659
SN 2192-2640
YR 2025
FD 2025-02-07
LK https://hdl.handle.net/10347/46395
UL https://hdl.handle.net/10347/46395
LA eng
NO Cardellini, J., Normak, K., Gerlt, M., Makasewicz, K., Seiffert, C., Capasso Palmiero, U., Ye, S., González Gómez, M. A., Piñero, Y., Rivas, J., Bongiovanni, A., Bergese, P., & Arosio, P. (2025). Microfluidics-Driven Manufacturing and Multiscale Analytical Characterization of Nanoparticle-Vesicle Hybrids. Advanced Healthcare Materials, 14(4). https://doi.org/10.1002/ADHM.202403264
NO This work was supported by the BOW project funded by the H2020-EU.1.2.2-FET Proactive program via Grant Agreement 952183. The authors acknowledge Prof. Jean-Christophe Leroux (ETH) for providing access to NTA, and the Florence Center for Electron Nanoscopy (FloCEN) at the University of Florence for the cryo-EM analysis.
DS Minerva
RD 23 may 2026