RT Journal Article
T1 Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatin
A1 Ribas-Maynou Jordi, 
A1 Muiño Otero, Rodrigo
A1 Tamargo, Carolina
A1 Yeste, Marc
K1 Cattle
K1 DNA damage
K1 Fertility
K1 Sperm
K1 Toroid linker regions
AB Sperm cryopreservation is widely used in the cattle industry, as it allows for disassociating the localization of sires and the collection of semen from the timing of artificial insemination. While freeze-thawing is known to impair sperm DNA integrity, whether the damage induced consists of single- (SSB) or double-strand breaks (DSB) has not been determined. In addition, no previous study has addressed if DNA breaks preferentially reside in specific genome regions such as those forming the toroid linker regions, or are rather spread throughout the regions linked to protamines. The main aim of the present work, therefore, was to elucidate the type and localization of the DNA damage generated by cryopreservation and to evaluate its impact on artificial insemination outcomes in cattle.
PB BioMed Central
SN 2049-1891
YR 2024
FD 2024-10-12
LK https://hdl.handle.net/10347/37725
UL https://hdl.handle.net/10347/37725
LA eng
NO Ribas-Maynou, J., Muiño, R., Tamargo, C. et al. Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatin. J Animal Sci Biotechnol 15, 140 (2024). https://doi.org/10.1186/s40104-024-01099-0
NO This research was supported by the Ministry of Science, Innovation and Universities, Spain (NextGeneration EU funds; María Zambrano Program 124/MTAI/22; and PID2020-113320RB-I00), the Agency for Management of University and Research Grants, Regional Government of Catalonia, Spain (2021-SGR-00900), and the Catalan Institution for Research and Advanced Studies (ICREA).
DS Minerva
RD 27 abr 2026