RT Journal Article
T1 Cometabolic enzymatic transformation of organic micropollutants under methanogenic conditions
A1 González Gil, Lorena
A1 Carballa Arcos, Marta
A1 Lema Rodicio, Juan Manuel
K1 Acetate kinase
K1 Anaerobic digestion
K1 Biotransformation
K1 Cometabolism
K1 Organic micropollutants
K1 Sewage sludge
AB Anaerobic digestion (AD) has been shown to have the biological potential to decrease concentrations of several organic micropollutants (OMPs) in sewage sludge. However, the mechanisms and factors behind these biotransformations, which are essential for elucidating the possible transformation products and to foster the complete removal of OMPs via operational strategies, remain unclear. Therefore, this study investigated the transformation mechanisms of 20 OMPs during the methanogenic step of AD with a focus on the role of acetate kinase (AK), which is a key enzyme in methane production. The results from lab-scale methanogenic reactors showed that this step accounts for much of the reported OMP biotransformation in AD. Furthermore, enzymatic assays confirmed that AK transforms galaxolide, naproxen, nonylphenol, octylphenol, ibuprofen, diclofenac, bisphenol A, and triclosan. Except for galaxolide, for which further studies are required to refine conclusions, the OMP’s chemical structure was a determinant for AK action because only compounds that contain a carboxyl or hydroxyl group and have moderate steric hindrance were enzymatically transformed, likely by phosphorylation. For these seven compounds, this enzymatic mechanism accounts for 10–90% of the measured methanogenic biotransformation, suggesting that other active enzymes of the AD process are also involved in OMP biotransformation
PB American Chemical Society
YR 2017
FD 2017-02-15
LK http://hdl.handle.net/10347/16674
UL http://hdl.handle.net/10347/16674
LA eng
NO Gonzalez-Gil, L., Carballa, M., & Lema, J. M. (2017). Cometabolic Enzymatic Transformation of Organic Micropollutants under Methanogenic Conditions. Environmental Science & Technology, 51(5), 2963-2971
NO This is a post-print version of the published article: Gonzalez-Gil, L., Carballa, M., & Lema, J. M. (2017). Cometabolic Enzymatic Transformation of Organic Micropollutants under Methanogenic Conditions. Environmental Science & Technology, 51(5), 2963-2971. https://doi.org/10.1021/acs.est.6b05549
NO This work was funded by Xunta de Galicia through the MicroDAN project (EM 2012/087) and by the Spanish government through the HOLSIA project (CTM2013-46750-R), a Ramón y Cajal contract (RYC-2012-10397) and an FPU Grant (FPU13/01255). The authors belong to CRETUS (AGRUP2015/02) and to the Galician Competitive Research Group (GRC 2013-032)
DS Minerva
RD 23 abr 2026