RT Journal Article
T1 Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin
A1 Vázquez Iglesias, Lorena
A1 Lostalé Seijo, Irene
A1 Martínez Costas, José Manuel
A1 Benavente Martínez, Francisco Javier
K1 Avian reovirus
K1 SigmaA
K1 Intracellular distribution
K1 Nuclear import
K1 CRM1-dependent export
K1 Nuclear matrix
K1 Nucleocytoplasmic shuttling
AB A comparative analysis of the intracellular distribution of avian reovirus (ARV) core protein sigmaA in cells of avian and mammalian origin revealed that, whereas the viral protein accumulates in the cytoplasm and nucleolus of avian cells, most sigmaA concentrates in the nucleoplasm of mammalian cells in tight association with the insoluble nuclear matrix fraction. Our results further showed that sigmaA becomes arrested in the nucleoplasm of mammalian cells via association with mammalian cell-specific factors and that this association prevents nucleolar targeting. Inhibition of RNA polymerase II activity, but not of RNA polymerase I activity, in infected mammalian cells induces nucleus-to-cytoplasm sigmaA translocation through a CRM1- and RanGTP-dependent mechanism, yet a heterokaryon assay suggests that sigmaA does not shuttle between the nucleus and cytoplasm. The scarcity of sigmaA in cytoplasmic viral factories of infected mammalian cells could be one of the factors contributing to limited ARV replication in mammalian cells
PB Elsevier
SN 0042-6822
YR 2012
FD 2012
LK http://hdl.handle.net/10347/18494
UL http://hdl.handle.net/10347/18494
LA eng
NO Vázquez-Iglesias, L., Lostalé-Seijo, I., Martínez-Costas, J., & Benavente, J. (2012). Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin. Virology, 432(2), 495-504. doi: 10.1016/j.virol.2012.07.007
NO This research was supported by grants from the Spanish Ministerio de Ciencia y Tecnología (BFU2007-61330/BMC) and from the Xunta de Galicia (08CSA009203PR). L. V-I. and I. L-S. were recipients of predoctoral fellowships from the FPI and FPU programs of the Spanish Ministerio de Ciencia y Tecnología
DS Minerva
RD 24 abr 2026