RT Journal Article
T1 State of art of micronuclei assay in exfoliative cytology as a clinical biomarker of genetic damage in oral carcinogenesis: A systematic review and meta-analysis
A1 Caponio, Vito Carlo Alberto
A1 Vieira e Silva, Fábio França
A1 Popolo, Francesco
A1 Giugliano, Sara
A1 Spizzirri, Francesca
A1 Lorenzo Pouso, Alejandro Ismael
A1 Padín Iruegas, María Elena
A1 Zhurakivska, Khrystyna
A1 Lo Muzio, Lorenzo
A1 López Pintor, Rosa María
K1 Micronucleus tests
K1 Tobacco products
K1 Risk factors
K1 Pesticides
K1 Mouth neoplasms
K1 Meta-analysis
AB Oral squamous cell carcinoma (OSCC) is the most common oral malignancy, often preceded by oral potentially malignant disorders (OPMDs). Currently, no clinical biomarker exists to predict malignancy, necessitating OPMD follow-up. Habits and environmental factors, such as smoking, and alcohol consumption, influence OSCC onset. Increased micronuclei (MNs) formation has been observed in the development of OSCC. Non-invasive diagnostic tests like exfoliative cytology offer painless and regular monitoring options. This study evaluates the impact of tobacco, alcohol, and pesticide exposure on MNs occurrence in exfoliative cytology-collected oral mucosal cells, assessing their potential as non-invasive biomarker for OSCC development prediction and monitoring in high-risk patients. Despite results from this meta-analysis supporting the existence of a stepwise increase from controls to patients with OPMD to OSCC, the translation of these findings into clinical practice is limited due to intra- and inter-individual heterogeneity, as well as methodological variability in MNs quantification. Various factors contribute to this heterogeneity, including demographic variables, methodological variability of different laboratories, staining techniques, sample collection location, and patient characteristics. All these points were discussed to provide further insights and improve standardization for future studies
PB Elsevier B.V.
YR 2024
FD 2024-06-22
LK https://hdl.handle.net/10347/40140
UL https://hdl.handle.net/10347/40140
LA eng
NO The research leading to these results has received funding from the European Union - NextGenerationEU through the Italian Ministry of University and Research under PNRR - M4C2-I1.3 Project PE_00000019 “HEAL ITALIA HEALTH EXTENDED ALLIANCE FOR INNOVATIVE THERAPIES, ADVANCED LAB-RESEARCH, AND INTEGRATED APPROACHES OF PRECISION MEDICINE” to Vito Carlo Alberto Caponio and Lorenzo Lo Muzio CUP D73C22001230006
DS Minerva
RD 23 abr 2026