RT Journal Article
T1 Comparative functional genomics identifies unique molecular features of EPSCs
A1 Malik, Vikas
A1 Zang, Ruge
A1 Fuentes Iglesias, Alejandro
A1 Huang, Xing
A1 Fidalgo Pérez, Miguel Ángel
K1 Genomics
K1 Molecular features
K1 EPSCs
K1 Extended pluripotent or expanded potential stem cells
K1 Stem cells
AB Extended pluripotent or expanded potential stem cells (EPSCs) possess superior developmental potential to embryonic stem cells (ESCs). However, the molecular underpinning of EPSC maintenance in vitro is not well defined. We comparatively studied transcriptome, chromatin accessibility, active histone modification marks, and relative proteomes of ESCs and the two well-established EPSC lines to probe the molecular foundation underlying EPSC developmental potential. Despite some overlapping transcriptomic and chromatin accessibility features, we defined sets of molecular signatures that distinguish EPSCs from ESCs in transcriptional and translational regulation as well as metabolic control. Interestingly, EPSCs show similar reliance on pluripotency factors Oct4, Sox2, and Nanog for self-renewal as ESCs. Our study provides a rich resource for dissecting the regulatory network that governs the developmental potency of EPSCs and exploring alternative strategies to capture totipotent stem cells in culture
PB EMBO Press
SN 2575-1077
YR 2022
FD 2022-08-12
LK https://hdl.handle.net/10347/40690
UL https://hdl.handle.net/10347/40690
LA eng
NO Vikas Malik, Ruge Zang, Alejandro Fuentes-Iglesias, Xin Huang, Dan Li, Miguel Fidalgo, Hongwei Zhou, Jianlong Wang Life Science Alliance Aug 2022, 5 (11) e202201608; DOI: 10.26508/lsa.202201608
NO MINECO España (BES-2017-082007)
DS Minerva
RD 22 abr 2026