RT Journal Article
T1 Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN
A1 Romero Bernárdez, Manuel
A1 Silistre, Hazel
A1 Lovelock, Laura
A1 Wright, Victoria J.
A1 Chan, Kok-Gan
A1 Hong, Kar-Wai
A1 Williams, Paul
A1 Cámara, Miguel
A1 Heeb, Stephen
AB Pseudomonads typically carry multiple non-identical alleles of the post-transcriptional regulator rsmA. In Pseudomonas aeruginosa, RsmN is notable in that its structural rearrangement confers distinct and overlapping functions with RsmA. However, little is known about the specificities of RsmN for its target RNAs and overall impact on the biology of this pathogen. We purified and mapped 503 transcripts directly bound by RsmN in P. aeruginosa. About 200 of the mRNAs identified encode proteins of demonstrated function including some determining acute and chronic virulence traits. For example, RsmN reduces biofilm development both directly and indirectly via multiple pathways, involving control of Pel exopolysaccharide biosynthesis and c-di-GMP levels. The RsmN targets identified are also shared with RsmA, although deletion of rsmN generally results in less pronounced phenotypes than those observed for ΔrsmA or ΔrsmArsmNind mutants, probably as a consequence of different binding affinities. Targets newly identified for the Rsm system include the small non-coding RNA CrcZ involved in carbon catabolite repression, for which differential binding of RsmN and RsmA to specific CrcZ regions is demonstrated. The results presented here provide new insights into the intricacy of riboregulatory networks involving multiple but distinct RsmA homologues.
PB Oxford University Press
YR 2018
FD 2018
LK https://hdl.handle.net/10347/38429
UL https://hdl.handle.net/10347/38429
LA eng
NO Manuel Romero, Hazel Silistre, Laura Lovelock, Victoria J Wright, Kok-Gan Chan, Kar-Wai Hong, Paul Williams, Miguel Cámara, Stephan Heeb, Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN, Nucleic Acids Research, Volume 46, Issue 13, 27 July 2018, Pages 6823–6840, https://doi.org/10.1093/nar/gky324
DS Minerva
RD 23 abr 2026