RT Journal Article
T1 Tackling proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress
A1 Franco Ruiz, Daniel
A1 Mato, Ariadna
A1 Salgado Castro, Francisco Javier
A1 López-Pedrouso, María
A1 Carrera, Mónica
A1 Bravo, Susana
A1 Parrado, María
A1 Gallardo, José Manuel
A1 Zapata Babío, José Carlos
K1 Pre-slaughter stress
K1 Biomarkers
K1 Bos taurus
K1 DFD meat
K1 Longissimus thoracis
K1 Proteome
K1 Relative change measure
AB Pre-slaughter stress has adverse effects on meat quality that can lead to the occurrence of Dark Firm Dry (DFD) meat in cattle. This study explores the previously uncharacterized proteome changes linked to pre-slaughter stress in the longissimus thoracis (LT) bovine muscle. Differential proteome profiles of DFD and normal (non-DFD) LT meat samples from male calves of the Rubia Gallega breed were assessed by 2-DE coupled to MS analysis (LC-MS/MS and MALDI TOF/TOF MS). A total of seven structural-contractile proteins (three different myosin light chain isoforms, two fast skeletal myosin light chain 2 isoforms, troponin C type 2 and cofilin-2) and three metabolism enzymes (triosephosphate isomerase, ATP synthase and beta-galactoside alpha-2,6-sialyltransferase) were found to have statistically significant differential abundance in sample groups. In addition, 2-DE in combination with the phosphoprotein-specific fluorescent dye Pro-Q DPS revealed that highly phosphorylated fast skeletal myosin regulatory light chain 2 isoforms underwent the most intense relative change in muscle conversion to DFD meat. Therefore, they appear to be the most sensitive biomarkers of stress just prior to slaughter in Rubia Gallega. Overall, these findings will facilitate a more integrative understanding of the biochemical processes associated with stress in cattle muscle and their effects in meat quality.
PB Elsevier
SN 1874-3919
YR 2015
FD 2015-06-03
LK https://hdl.handle.net/10347/39082
UL https://hdl.handle.net/10347/39082
LA eng
NO Franco, D., Mato, A., Salgado, F. J., López-Pedrouso, M., Carrera, M., Bravo, S., ... & Zapata, C. (2015). Tackling proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress. Journal of proteomics, 122, 73-85.
DS Minerva
RD 23 abr 2026