RT Journal Article
T1 Effect of Resveratrol on Oxygen Consumption by Philasterides dicentrarchi, a Scuticociliate Parasite of Turbot
A1 Morais, Pedro
A1 Piazzon, Carla
A1 Lamas Fernández, Jesús
A1 Mallo Seijas, Natalia
A1 Leiro, José Manuel
A1 Leiro Vidal, José Manuel
K1 Philasterides dicentrarchi
K1 resveratrol
K1 mitochondrial metabolism
K1 pro-oxidant
K1 reactive oxygen species
K1 oxygen consumption
K1 antiparasitic
AB The phytoalexin resveratrol (RESV) displays antiparasitic activity against Philasterides dicentrarchi, a scuticociliate pathogen of turbot, and causes oxidative stress, inhibition of antioxidant enzyme activity and morphological alterations in the parasite mitochondria. In this study, we analysed the mitochondrial biology of P. dicentrarchi and assessed the effect of RESV on mitochondrial metabolism. We found that RESV caused dose-dependent inhibition of mitochondrial electron transport and O2 consumption in ciliates permeabilized with digitonin. Although the RESV molecule has a high capacity for antiradical and antioxidant activity, it induced a high level of pro-oxidant activity against the ciliate, thus causing a significant increase in intracellular ROS production. The increased ROS production was accompanied by mitochondrial collapse and dysfunction of mitochondrial membrane potential (ΔΨm) and by a significant increase in intracellular Ca+2 levels. RESV inhibited parasite growth in a similar way to antimycin A, an inhibitor of mitochondrial electron transport and ROS generator. The findings confirm the mitochondria as a target in the potential development of effective antiparasitic treatments.
PB Elsevier
SN 1434-4610
YR 2013
FD 2013-03
LK https://hdl.handle.net/10347/45942
UL https://hdl.handle.net/10347/45942
LA eng
NO Morais, P., Piazzon, C., Lamas, J., Mallo, N., & Leiro, J. M. (2013). Effect of resveratrol on oxygen consumption by philasterides dicentrarchi, a scuticociliate parasite of turbot. Protist, 164(2), 206–217. doi:10.1016/j.protis.2012.07.002
DS Minerva
RD 24 abr 2026