RT Journal Article
T1 Plasmonic-assisted thermocyclizations in living cells using metal−organic framework based nanoreactors
A1 Carrillo Carrión, Carolina
A1 Martínez González, Raquel
A1 Polo Tobajas, Ester
A1 Tomás Gamasa, María
A1 Destito, Paolo
A1 Ceballos Guzmán, Manuel
A1 Pelaz García, Beatriz
A1 López García, Fernando
A1 Mascareñas Cid, José Luis
A1 Pino González de la Higuera, Pablo Alfonso del
K1 Bio-orthogonal chemistry
K1 Nanocomposites
K1 Thermoplasmonics
K1 Intracellular thermocyclization
K1 Thermolabile protecting groups
AB We describe a microporous plasmonic nanoreactor to carry out designed near-infrared (NIR)-driven photothermal cyclizations inside living cells. As a proof of concept, we chose an intramolecular cyclization that is based on the nucleophilic attack of a pyridine onto an electrophilic carbon, a process that requires high activation energies and is typically achieved in bulk solution by heating at ∼90 °C. The core–shell nanoreactor (NR) has been designed to include a gold nanostar core, which is embedded within a metal–organic framework (MOF) based on a polymer-stabilized zeolitic imidazole framework-8 (ZIF-8). Once accumulated inside living cells, the MOF-based cloak of NRs allows an efficient diffusion of reactants into the plasmonic chamber, where they undergo the transformation upon near-IR illumination. The photothermal-driven reaction enables the intracellular generation of cyclic fluorescent products that can be tracked using fluorescence microscopy. The strategy may find different type of applications, such as for the spatio-temporal activation of prodrugs
PB ACS
SN 1936-0851
YR 2021
FD 2021
LK http://hdl.handle.net/10347/27035
UL http://hdl.handle.net/10347/27035
LA eng
NO ACS Nano 2021, 15, 16924−16933
NO The authors thank the financial support of the MCIN/AEI (PID2020-119206RB-I00, PID2019-108624RB-I00, CTQ2017-84767-P, RYC-2017-23457, RYC-2019-028238-I, RTI2018-093813-J-I00), the Xunta de Galicia (ED431F 2017/02, 2021-CP054, ED431C-2021/25, Centro Singular de Investigación de Galicia Accreditation 2019−2022, and ED431G 2019/03), the European Union (European Regional Development Fund − ERDF; H2020-MSCA-IF grant agreement no. 749667; H2020-MSCA-ITN grant agreement no. 860942; H2020-FET-Open grant agreement No. 899612; and INTERREG V-A Spain−Portugal, project 0624_2IQBIONEURO_6_E), and the European Research Council (starting grant no. 950421, advanced grant no. 340055). The support of the orfeo-cinqa network (CTQ2016-81797-REDC) is also kindly acknowledged
DS Minerva
RD 22 abr 2026