RT Journal Article
T1 Revisiting the taxonomy of the genus Arcobacter: Getting order from the chaos
A1 Pérez Cataluña, Alba
A1 Salas Massó, Nuria
A1 López Diéguez, Ana Belén
A1 Balboa Méndez, Sabela
A1 Lema Blanco, Alberto
A1 López Romalde, Jesús
A1 Figueras, Maria J.
K1 Arcobacter
K1 Aliiarcobactergen. nov.
K1 Pseudoarcobactergen. nov.
K1 Haloarcobactergen. nov.
K1 Malacobactergen. nov.
K1 Poseidonibactergen. nov.
K1 Taxonomic criteria
AB Since  the  description  of  the  genusArcobacterin  1991,  a  total  of  27  species  havebeen described, although some species have shown 16S rRNA similarities below 95%,which  is  the  cut-off  that  usually  separates  species  that  belong  to  different  genera.The  objective  of  the  present  study  was  to  reassess  the  taxonomy  of  the  genusArcobacterusing information derived from the core genome (286 genes), a MultilocusSequence Analysis (MLSA) with 13 housekeeping genes, as well as different genomicindexes like Average Nucleotide Identity (ANI),in silicoDNA–DNA hybridization (isDDH),Average  Amino-acid  Identity  (AAI),  Percentage  of  Conserved  Proteins  (POCPs),  andRelative Synonymous Codon Usage (RSCU). The study included a total of 39 strainsthat represent all the 27 species included in the genusArcobactertogether with 13strains that are potentially new species, and the analysis of 57 genomes. The differentphylogenetic analyses showed that theArcobacterspecies grouped into four clusters.In addition,A. lekithochrousand the candidatus species ‘A. aquaticus’ appeared, asdidA. nitrofigilis,the type  species of the genus,  in separate branches.  Furthermore,the genomic indices ANI andisDDH not only confirmed that all the species were well-defined,  but  also  the  coherence  of  the  clusters.  The  AAI  and  POCP  values  showedintra-cluster  ranges  above  the  respective  cut-off  values  of  60%  and  50%  describedfor  species  belonging  to  the  same  genus.  Phenotypic  analysis  showed  that  certaintest  combinations  could  allow  the  differentiation  of  the  four  clusters  and  the  threeorphan  species  established  by  the  phylogenetic  and  genomic  analyses.  The  originof  the  strains  showed  that  each  of  the  clusters  embraced  species  recovered  froma  common  or  related  environment.  The  results  obtained  enable  the  division  of  thecurrent  genusArcobacterin  at  least  seven  different  genera,  for  which  the  namesArcobacter,Aliiarcobactergen. nov.,Pseudoarcobactergen. nov.,Haloarcobactergen.nov.,Malacobactergen. nov.,Poseidonibactergen. nov., and Candidate ‘Arcomarinus’gen. nov. are proposed
PB Frontiers Media
YR 2018
FD 2018
LK http://hdl.handle.net/10347/23171
UL http://hdl.handle.net/10347/23171
LA eng
NO Pérez-Cataluña A, Salas-Massó N, Diéguez AL, Balboa S, Lema A, Romalde JL and Figueras MJ (2018) Revisiting the Taxonomy of the Genus Arcobacter: Getting Order From the Chaos. Front. Microbiol. 9:2077. doi: 10.3389/fmicb.2018.02077
NO This work was supported in part by Grants JPIW2013-69095-C03-03 from the Ministerio de Economía y Competitividad (MINECO), AQUAVALENS of the Seventh Framework Program (FP7/2007-2013) grant agreement 311846 from the European Union and AGL2013-42628-R and AGL2016-77539-R (AEI/FEDER UE) from the Agencia Estatal de Investigación (Spain)
DS Minerva
RD 22 abr 2026