RT Journal Article
T1 Surface monitoring of L. monocytogenes by real‑time fluorescence and colorimetric LAMP
A1 Ábalo, Maruxa
A1 Lamas Freire, Alexandre
A1 Teixeira, Carla
A1 Prado Rodríguez, Marta
A1 Garrido Maestu, Alejandro
K1 Listeria monocytogenes
K1 Surface analysis
K1 Loop-mediated isothermal amplification
K1 Naked-eye detection
K1 Stainless steel
AB Listeria monocytogenes is a major foodborne pathogen affecting developing, and developed countries. The analysis of food contact surfaces in food industries is key for better controlling this pathogen. The current study focused on the development, optimization, and evaluation of a rapid and simple method for the detection of L. monocytogenes on stainless steel surfaces, suitable for decentralized setups, taking advantage of Loop-mediated isothermal amplification (LAMP). This was accomplished using a general pre-enrichment broth (TSB), with a simple DNA extraction based on a chelating resin, and final isothermal amplification. Two different detection strategies were tested, real-time fluorescence and naked-eye colorimetric, which were evaluated after 5, 7, and 24 h of pre-enrichment. Regardless the detection chemistry selected, after 5–7 h of preenrichment, 103 –104 CFU/cm2 were needed to obtain a positive result, while after 24 h, it was possible to detect concentrations below 10 CFU/cm2. Within each given time, all the performance parameters calculated, relative sensitivity, specificity, and accuracy, reached values higher than 80–90%; likewise, a Cohen’s k of concordance with a culture-based approach higher than 0.8. Overall, the most sensitive assay can be performed in roughly 25 h. This time-to-result outperforms commercial kits with the added value of specifically detecting L. monocytogenes instead of Listeria spp.
PB Springer Nature
SN 0175-7598
YR 2024
FD 2024-11-12
LK https://hdl.handle.net/10347/43497
UL https://hdl.handle.net/10347/43497
LA eng
NO Applied Microbiology and Biotechnology (2024) 108:510 https://doi.org/10.1007/s00253-024-13318-9
NO Agenda SMARTgNOSTICS – Global Testing & Diagnostics Solutions for antimicrobial resistances C644915155-00000024
DS Minerva
RD 23 abr 2026