RT Journal Article
T1 A microsatellite panel for mating system analysis and broodstock management of captive long-snouted seahorse Hippocampus guttulatus
A1 López Martínez, Almudena
A1 Gómez Pardo, María Belén
A1 Planas, Miquel
A1 Quintas, Patricia
A1 Martínez Portela, Paulino
A1 Bouza Fernández, María Carmen
K1 Hippocampus guttulatus
K1 Microsatellites
K1 Parentage analysis
K1 Mating system
K1 Captive breeding
AB We characterized a microsatellite-based panel for parentage analysis in Hippocampus guttulatus using families obtained under captive conditions. Offspring obtained by natural breeding from 49 founder breeders in different aquaria were genotyped for six selected loci. A theoretical exclusion power for parentage inference close to one was obtained from the broodstock, which enabled us to trace back all offspring to a single parental pair with high confidence, under the simple mating scenario managed. The family analysis revealed a very low percentage of false exclusions due to genotyping errors or null alleles (0.9%) and conformance to Mendelian segregation for all loci. Their potential for parentage analysis was confirmed in a more complex scenario, using all breeders as parental candidates, with over 95% of offspring being assigned to the same single pair. Parentage data revealed a monogamous mating system for H. guttulatus in captivity. These loci constitute a useful panel for genealogical traceability and also for investigating relatedness among founders to assist with captive breeding for H. guttulatus
PB Elsevier
SN 0044-8486
YR 2012
FD 2012
LK http://hdl.handle.net/10347/26153
UL http://hdl.handle.net/10347/26153
LA eng
NO Aquaculture 356-357(1): 153-157 (2012)
NO This work was supported by the Spanish Government (CGL2005-05927-C03-01/-03; CGL2009-08386) and the Regional Government of Galicia (09MDS022402PR)
DS Minerva
RD 24 abr 2026