RT Journal Article
T1 Epitype-inducing temperatures drive DNA methylation changes during somatic embryogenesis in the long-lived gymnosperm Norway spruce
A1 Viejo Somoano, Marcos
A1 Tengs, Torstein
A1 Yakovlev, Igor
A1 Cross, Hugh
A1 Krokene, Paal
A1 Olsen, Jorunn E.
A1 Fossdal, Carl Gunnar
K1 Embryogenesis
K1 DNA methylation
K1 Epigenetic memory
K1 Epigenetic machinery
K1 Spruce
AB An epigenetic memory of the temperature sum experienced during embryogenesis is part of the climatic adaptation strategy of the long-lived gymnosperm Norway spruce. This memory has a lasting effect on the timing of bud phenology and frost tolerance in the resulting epitype trees. The epigenetic memory is well characterized phenotypically and at the transcriptome level, but to what extent DNA methylation changes are involved have not previously been determined. To address this, we analyzed somatic epitype embryos of Norway spruce clones produced at contrasting epitype-inducing conditions (18 and 28°C). We screened for differential DNA methylation in 2744 genes related mainly to the epigenetic machinery, circadian clock, and phenology. Of these genes, 68% displayed differential DNA methylation patterns between contrasting epitype embryos in at least one methylation context (CpG, CHG, CHH). Several genes related to the epigenetic machinery (e.g., DNA methyltransferases, ARGONAUTE) and the control of bud phenology (FTL genes) were differentially methylated. This indicates that the epitype-inducing temperature conditions induce an epigenetic memory involving specific DNA methylation changes in Norway spruce.
PB Frontiers Media
SN 1664-462X
YR 2023
FD 2023-07-20
LK https://hdl.handle.net/10347/42943
UL https://hdl.handle.net/10347/42943
LA eng
NO Viejo M, Tengs T, Yakovlev I, Cross H, Krokene P, Olsen JE and Fossdal CG (2023) Epitype-inducing temperatures drive DNA methylation changes during somatic embryogenesis in the long-lived gymnosperm Norway spruce. Front. Plant Sci. 14:1196806. doi: 10.3389/fpls.2023.1196806
NO This work was supported by the Research Council of Norway through FRIMEDBIO Grant#240766/F20, TOPPFORSK Grant#249958/F20 and FRIPRO Grant#325671).
DS Minerva
RD 23 abr 2026