RT Journal Article
T1 Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
A1 Costa, Pedro
A1 Pereira, Carla
A1 Oliveira, Vanessa
A1 Gomes, Newton C.M.
A1 López Romalde, Jesús
A1 Almeida, Adelaide
K1 Bacteriophages
K1 Pathogenic bacteria
K1 Bivalve consumption
K1 Phage biocontrol
AB In the present study, five new bacteriophages (or phages) were characterized, and their efficacy in controlling pathogenic bacteria—Escherichia coli, Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, and Vibrio parahaemolyticus—associated with bivalve consumption was evaluated. The isolated phages include both siphovirus [vB_EcoS_UALMA_PCEc3 (PCEc3), vB_SeTS_UALMA_PCST1 (PCST1), and vB_VpaS_UALMA_PCVp3 (PCVp3)] and myovirus [vB_SeEM_UALMA_PCSE1 (PCSE1) and vB_AhyM_UALMA_PCAh2 (PCAh2)] morphotypes. Four phages are safe for bacterial control, with only one (PCAh2) showing potential lysogenic characteristics. All phages exhibited a narrow host range, capable of infecting up to six additional bacterial strains besides their original host, and four could infect the host bacteria of other phages. Adsorption rates ranged from 24% and 98% within 1 h. One-step growth assays revealed different latent periods, ranging from 10 to 120 min, and low to average burst sizes, ranging from 7.60 to 83.97 PFU/mL. Generally, increasing the multiplicity of infection (MOI) enhanced phage efficiency significantly. All phages effectively reduced the bacterial load of their respective hosts, achieving maximum reductions between 3.73 and 5.57 log CFU/mL within 10 h of treatment. These results suggest that phage biocontrol can be an effective alternative to combat pathogenic bacteria associated with bivalve consumption.
PB Elsevier
YR 2025
FD 2025
LK https://hdl.handle.net/10347/42385
UL https://hdl.handle.net/10347/42385
LA eng
NO Costa, P., Pereira, C., Oliveira, V., Gomes, N. C. M., Romalde, J. L., & Almeida, A. (2025). Characterising phages for the control of pathogenic bacteria associated with bivalve consumption. International Journal of Food Microbiology, 432, pp. 1-14. https://doi.org/10.1016/j.ijfoodmicro.2025.111096
NO Through national funds, we acknowledge financial support to CESAM by FCT/MCTES (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/2020). Pedro Costa was supported in the form of a PhD grant (PD/BD/150360/2019) and Carla Pereira by a Junior Research contract (DOI: 10.54499/CEECIND/03974/2017/CP1459/CT0022), financed by national funds through the FCT - Foundation for Science and Technology, I.P. Vanessa Oliveira was funded by National funds (OE), through FCT, IP., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of article 23 of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19 (DOI 10.54499/DL57/2016/CP1482/CT0109).
DS Minerva
RD 30 abr 2026