RT Journal Article
T1 Bioelectronics-on-a-chip for cardio myoblast proliferation enhancement using electric field stimulation
A1 Aragón Beloso, Ángel Luis
A1 Cebro Márquez, María
A1 Pérez Trigo, Eliseo
A1 Pazos Álvarez, Antonio
A1 Lage Fernández, Ricardo
A1 González Juanatey, José Ramón
A1 Moscoso Galán, Isabel
A1 Bao Varela, María del Carmen
A1 Nieto García, Daniel
K1 Cell electrostimulation
K1 Bioelectronics chip
K1 Laser microfabrication
K1 Cell culture
AB Background:Cardio myoblast generation from conventional approaches is laborious and time-consuming. We present a bioelectronics on-a-chip for stimulating cells cardio myoblast proliferation during culture.Method:The bioelectronics chip fabrication methodology involves two different process. In the first step, an aluminum layer of 200 nm is deposited over a soda-lime glass substrate using physical vapor deposition and selectively removed using a Q-switched Nd:YVO4 laser to create the electric tracks. To perform the experiments, we developed a biochip composed of a cell culture chamber fabricated with polydimethylsiloxane (PDMS) with a glass coverslip or a cell culture dish placed over the electric circuit tracks. By using such a glass cover slip or cell culture dish we avoid any toxic reactions caused by electrodes in the culture or may be degraded by electrochemical reactions with the cell medium, which is crucial to determine the effective cell-device coupling.Results:The chip was used to study the effect of electric field stimulation of Rat ventricular cardiomyoblasts cells (H9c2). Results shows a remarkable increase in the number of H9c2 cells for the stimulated samples, where after 72 h the cell density double the cell density of control samples.Conclusions:Cell proliferation of Rat ventricular cardiomyoblasts cells (H9c2) using the bioelectronics-on-a-chip was enhanced upon the electrical stimulation. The dependence on the geometrical characteristics of the electric circuit on the peak value and homogeneity of the electric field generated are analyzed and proper parameters to ensure a homogeneous electric field at the cell culture chamber are obtained. It can also be observed a high dependence of the electric field on the geometry of the electrostimulator circuit tracks and envisage the potential applications on electrophysiology studies, monitoring and modulate cellular behavior through the application of electric fields
PB Springer Nature
SN 2055-7124
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23456
UL http://hdl.handle.net/10347/23456
LA eng
NO Aragón, Á., Cebro-Márquez, M., Perez, E. et al. Bioelectronics-on-a-chip for cardio myoblast proliferation enhancement using electric field stimulation. Biomater Res 24, 15 (2020). https://doi.org/10.1186/s40824-020-00195-2
NO This work was partially supported by Mineco through the projects FIS 2015–71933-REDT and RTI 2018–097063-B-I00, Consellería de Educación Program for Development of a Strategic Grouping in Materials – AeMAT Grant No. ED431E2018/08, Xunta de Galicia ref. ED431B2017/64. Xunta de Galicia, Spain, under Galician Programme for Research Innovation and Growth 2011–2015 (I2C Plan)
DS Minerva
RD 23 abr 2026