RT Journal Article
T1 Ciprofloxacin biotransformation during anaerobic digestion: Major transformation products elucidation and environmental proteomics
A1 Trueba Santiso, Alba María
A1 Rivadulla Cora, Matías
A1 Casabella Font, Oriol
A1 Cuervo Lumbaque, Elisabeth
A1 Radjenovic, Jelena
A1 Omil Prieto, Francisco
A1 Suárez Martínez, Sonia
K1 Anaerobic digestion
K1 Ciprofloxacin
K1 Biotransformation
K1 TPs identification
K1 Metaproteomics
AB Anaerobic membrane bioreactors (AnMBR) ar a promising alternative for treating high-load sewage, like black water (BW), due to the energy saving (biogas production and lack of aeration). Antibiotics like ciprofloxacin (CIP) are expected to be more concentrated than in a typical urban sewage matrix. Its fate under these conditions remains poorly understood. To broaden this understanding, we combined analysis of transformation products (TPs) and proteomics on 26 batch experiments inoculated with an AnMBR sludge, fed with volatile fatty acids (VFAs) and spiked with CIP. Methanogenic activity was not affected by CIP and the removal was higher than 88 % in all cases. Abiotic controls indicated the removal was mainly driven by sorption processes. However, at the lower concentrations tested (0.1 and 0.5 mg L−1) biotransformation also occurred. A total of six TPs were identified, some only present in low concentrations while one of them only appeared at the highest concentrations, pointing to hydroxylation and loss of the cyclopropyl ring as the main biological transformations. Methanothrix and Methanosaeta were the main methanogens and not negatively affected by CIP, except at 2 mg CIP. L−1 suggesting an inhibitory threshold. Environmental proteomics pointed to the key role of dehydrogenases under methanogenic anaerobic conditions.
PB Elsevier
SN 0304-3894
YR 2025
FD 2025-07-06
LK https://hdl.handle.net/10347/44085
UL https://hdl.handle.net/10347/44085
LA eng
NO Trueba-Santiso, A., Rivadulla, M., Casabella-Font, O., Cuervo-Lumbaque, E., Radjenovic, J., Omil, F., & Suárez, S. (2025). Ciprofloxacin biotransformation during anaerobic digestion: Major transformation products elucidation and environmental proteomics. Journal of Hazardous Materials, 495, 139159. 10.1016/j.jhazmat.2025.139159
NO This research was supported by the Spanish State Research Agency (EEA) through ARES (PID2022–141203OB-I00) and PRESAGE (PCI2021–121990) projects. O. Casabella-Font acknowledges funding from the Secretariat of Universities and Research from Generalitat de Catalunya and the European Social Fund for his FI fellowship (2023 FI-3 00122). E. Cuervo-Lumbaque has received funding from the European Union’s Horizon Europe research and innovation program under grant agreement No. 101062078. The authors acknowledge funding from the Galician Competitive Research Group (GRC)_ ED431C-2021/37 as well as the Economy and Knowledge Department of the Catalan Government through a Consolidated Research Group (ICRA-TECH - 2021 SGR 01283). We also acknowledge the support from Fundaci´o I-CERCA through the CERCAGINYS program, funded by the Ministerio de Ciencia e Innovaci´on (MICINN, Spanish Government). The graphical abstract was created in BioRender. Balboa, S. (2025) https://BioRender.co m/v6lf9hn.
DS Minerva
RD 28 abr 2026