RT Journal Article T1 Tetrodotoxins Occurrence in Non-Traditional Vectors of the North Atlantic Waters (Portuguese Maritime Territory, and Morocco Coast) A1 Silva, Marisa A1 Rodríguez Filgueiras, Inés A1 Barreiro, Aldo A1 Kaufmann, Manfred A1 Neto, Ana Isabel A1 Hassouani, Meryem A1 Sabour, Brahim A1 Alfonso Rancaño, María Amparo A1 Botana López, Luis Miguel A1 Vasconcelos, Vitor K1 Tetrodotoxin K1 New vectors K1 North Atlantic waters AB Tetrodotoxin (TTX) is a potent alkaloid typically from tropical ecosystems, but in the lastdecade its presence has been more pronounced in the temperate waters of the Atlantic. In its lastscientific opinion, the European Food Safety Authority (EFSA) stressed the need for data regardingTTX prevalence in European waters. To address EFSA’s concerns, benthic organisms such as mollusks,crustaceans, echinoderms and fish with different feeding habits were collected along the Portuguesecontinental coast, islands (São Miguel, Azores, and Madeira) and the northwestern Moroccan coast. Atotal of 165 samples were analyzed by ultra high performance liquid chromatography high resolutionmass spectrometry (UHPLC-HRMS) and ultra high performance chromatography mass spectrometry(UHPLC-MS/MS). Geographical tendencies were detected as follows, by descending order: S. MiguelIsland (Azores), Moroccan coast, Madeira Island and Portuguese continental coast. The toxin amountsdetected were significant, above the Dutch limit value established in 2017, showing the importanceand the need for continuity of these studies to gain more knowledge about the prevalence of thesetoxins, unraveling new vectors, in order to better assess human health risk. This work represents ageneral overview of new TTX bearers (7) most of them in gastropods (Patella depressa, Nucella lapillus,Onchidella celtica and Aplysia depilans), followed by echinoderms (Echinus esculentus and Ophidiasterophidianus) and puffer fish Sphoeroides marmoratus. PB MDPI YR 2019 FD 2019 LK http://hdl.handle.net/10347/21336 UL http://hdl.handle.net/10347/21336 LA eng NO Silva, M.; Rodríguez, I.; Barreiro, A.; Kaufmann, M.; Neto, A.I.; Hassouani, M.; Sabour, B.; Alfonso, A.; Botana, L.M.; Vasconcelos, V. Tetrodotoxins Occurrence in Non-Traditional Vectors of the North Atlantic Waters (Portuguese Maritime Territory, and Morocco Coast). Toxins 2019, 11, 306 NO This research was partially funded by the Portuguese Foundation of Science and Technology (FCT)project UID/Multi/04423/2013 and by the projects ALERTOXNET (EAPA_317/2016), funded by the Interreg Atlanticprogram. The research leading to these results has received funding from the following FEDER cofunded-grants.From Conselleria de Cultura, Educacion e Ordenación Universitaria, Xunta de Galicia, 2017 GRC GI-1682 (ED431C2017/01). From CDTI and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad,AGL2014-58210-R, AGL2016-78728-R (AEI/FEDER, UE), ISCIII/PI16/01830 and RTC-2016-5507-2, ITC-20161072.From European Union POCTEP 0161-Nanoeaters -1-E-1, Interreg Agritox EAPA-998-2018. Additional fundingwas provided by National Funds through FCT—Fundação para a Ciência e a Tecnologia, under the projectsUID/BIA/00329/2013, 2015 - 2018 and UID/BIA/00329/2019 DS Minerva RD 24 abr 2026