RT Dissertation/Thesis
T1 Polymeric nanocapsules for the ocular delivery of siRNA
A1 Mendes Saraiva, Sofia
K1 Nanomedicine
K1 protamine nanocapsules
K1 siRNA
AB The development of siRNA-based therapies for the treatment of severe ocular diseases is being proposed as a promising therapeutic option for the treatment of severe ocular diseases. Unfortunately, the capacity of siRNA molecules to overcome the multiple protective barriers of the eye is very limited. The specific objective of this work was to develop multiplayer protamine NCs loading high amounts of siRNA (up to 5 mg/mL) for ocular topical administration. Different preparation methods and compositions were studied for the development of NCs complying with the requirements of drug loading and release and also in terms of stability. Among the multiple formulations developed, one of them led to acceptable silencing results in vitro and showed the ability to interact efficiently with the rabbits’ corneal epithelium.
YR 2019
FD 2019
LK http://hdl.handle.net/10347/20532
UL http://hdl.handle.net/10347/20532
LA eng
DS Minerva
RD 23 abr 2026