RT Journal Article T1 Understanding the effect of trace elements supplementation on volatile fatty acids production from proteins A1 Bevilacqua, Riccardo A1 Regueira López, Alberte A1 Mauricio Iglesias, Miguel A1 Lema Rodicio, Juan Manuel A1 Carballa Arcos, Marta K1 Amino acid degradation K1 Biorefinery K1 Carboxylate platform K1 Micronutrients K1 Mixed-culture fermentation AB This investigation studies the impact of trace elements supplementation on protein anaerobic conversion into volatile fatty acids (VFAs). Two continuous reactors were operated with two model substrates, casein and gelatin, at acid (pH 5) and neutral (pH 7) conditions, with the addition of macro and micronutrients. Micronutrients increased the acidification degree of both proteins from 40% to 50% to more than 60% only at pH 7, which was consistent with a greater amino acid consumption at neutral conditions. Furthermore, trace elements modified the process selectivity, promoting valeric acids production and other variations dependant on protein composition. Isomerisation and chain elongation processes were identified as a consequence of the observed deviations between amino acid consumption and VFA production. Overall, this study demonstrated that the supplementation of micronutrients can be useful to enhance and steer the anaerobic fermentation of protein-rich streams PB Elsevier YR 2021 FD 2021 LK http://hdl.handle.net/10347/27647 UL http://hdl.handle.net/10347/27647 LA eng NO Journal of Environmental Chemical Engineering 9 (2021) 105934. https://doi.org/10.1016/j.jece.2021.105934 NO This project has received funding from the Spanish Government through the European Union’s ERA-IB programme under grant agreement number PCIN-2016-102 and from the Spanish Ministry of Education (FPU14/05457). The authors belong to a Galician Competitive Research Group (ED431C2017/029; ED431E 2018/01), co-funded by Xunta de Galicia (Spain) and the European Regional Development Fund (ERDF-EU) DS Minerva RD 28 abr 2026