RT Journal Article
T1 Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy
A1 Pena Rodríguez, Eloy
A1 García Berrocoso, Teresa
A1 Vázquez Fernández, Ezequiel
A1 Otero Espinar, Francisco Javier
A1 Abian Moñux, Joaquín
A1 Fernández Campos, Francisco
K1 MALDI-MSI
K1 Confocal Raman
K1 Skin
K1 Nanoparticle
K1 Semiquantitative
K1 Biodistribution
AB Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 μm) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions
PB Elsevier
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30616
UL http://hdl.handle.net/10347/30616
LA eng
NO International Journal of Pharmaceutics 636 (2023) 122808
NO This research received funding from the Generalitat de Catalunya Industrial Doctorate program of Eloy Pena Rodríguez, expedient number 2019 DI 1989691. Teresa García-Berrocoso holds a grant PTA2018-016332-I funded by MCIN/AEI10.13039/501100011033.
DS Minerva
RD 24 abr 2026